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Ovary may be the synthetic position of vitellin.

真水狼蛛的卵黄蛋白可能是由卵巢自身合成的。

Studies on ultrastructure indicate that the follicular cells have the function to filter the extraneous substances, and they can also synthesize some vitellin materials toprovide nourishment which is needed by the development of the oocytc.

超微结构研究表明,滤泡上皮细胞除具筛选物质的作用外,还可合成某些卵黄物质,为卵母细胞的发育提供营养。

The content of essential amino acid was 57.48%, which was little higher than that of non-essential amino acid. The amino acid with the highest content in vitellin was Glu, its content was 15.26%; while contents of Trp and Met were very low, only 0.50% and 0.11%, respectively.

卵黄蛋白的氨基酸组成和含量分析发现,其必需氨基酸总量占57.48%,略高于非必需氨基酸,其中谷氨酸含量最高,为15.26%;色氨酸和蛋氨酸含量较低,分别为0.50%和0.11%。

The results indicated that vitellin had a molecular weight of approximately 294.81±40.70 kD and was composed of two subunits, whose molecular weights were 144.68±0.03 kD and 51.23±0.27 kD respectively.

PAGE和SDS-PAGE实验表明,龟纹瓢虫卵黄蛋白分子量为294.81±40.70kD,并由分子量分别为144.68±0.03kD和51.23±0.27kD的两种亚基组成。

This paper was mainly working on the biochemical changes (water, carbohydrate, lipid, fatty acid, protein, amino acid) during embryonic development of Cherax quadricarinatus. At the same time, we especially worked on the yolk protein-vitellin in two aspects: 1. Isolation, purification and characterization of vitellin from the ovary of female Cherax quadricarinatus.

本文对红螯螯虾胚胎发育过程中的主要生化物质(水分、碳水化合物、脂类、脂肪酸、蛋白质、氨基酸)进行了测定,特别是对胚胎发育过程中主要的营养物质——卵黄磷蛋白的结构和含量变化做了细致的研究,同时讨论了这些物质的变化与红螯螯虾胚胎发育形态发生的相关性。

The soluble protein content decreased during embryonic development. Vitellin was a main component of soluble proteins. Besides, there were other soluble protein subunits such as 243 ku, 181 ku, 67 ku, 54 ku and 31 ku proteins.

可溶蛋白的含量在胚胎发育过程中逐渐降低;在成熟卵细胞和胚胎期,可溶蛋白在组成上以89ku和100ku的卵黄磷蛋白(Vitellin, Vn)为主,同时还存在243ku、181ku、67ku、54ku和31ku等其他一些蛋白亚基。40ku蛋白亚基仅出现在成熟卵细胞中,推测可能参与执行了特定的生殖功能。

Yolk protens of Bombyx mandarina are also composed of vitellin, egg specific proteins and 30K proteins on the base of SDS-PAGE of egg subsracts of Bombyx mori and Bombyx mandarina.

通过家蚕与野桑蚕卵的内容物的SDS-PAGE,分析了野桑蚕卵黄蛋白主要有卵黄磷蛋白,卵雌异性蛋白和30K蛋白组成。

Both the proteins components contained in haemolymph, fat body and ovary and the kenetics of the vitellogenin in haemolymph and ovary were analyzed by SDS—polyacrylamid electrophoresis, Ouchterlony plate tests and Rocket—immuno—electrophoresis. The results showed that the vitellogenesis initiated in adult stage and the Vg in haemolymph was detected firstly at 48h after eclosion. The titer of Vg in haemolymph raised rapidly in following 3 days and almost at the same time the vitellin appeared in oocytes. It was taken about 4—7 days after eclosion for the first eggs to grow up to the matured statics and to be laid out.

SDS-PAGE,双向免疫扩散和RIE分析粘虫生殖周期中血淋巴,脂肪体和卵巢中蛋白质谱带以及Vg滴度的变化,结果表明,Vg发生始于成虫期,Vg在血淋巴中的首现时间在羽化的24小时以后,以后连续三天血淋巴中的Vg滴度迅速上升,与此同时,开始沉积卵黄,卵母细胞逐渐发育长大,到成熟排出体外时大约历时4-7天。

GSTM, GST1 and GST2 localized extensively in the vitelline glands and ova of adult C.

GSTM、GST1和GST2主要位于成虫的卵黄腺和虫卵。

With the internal and external restraining and killing experiment of E. tenella, effects of specific high immunized vitelline antibody on E.

tenella进行体内外抑制和杀灭实验,观察特异性高免卵黄抗体对E。

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