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It is concluded that (1) there are no relationships between fusion rate and relativeness of the recipient cytoplasm to nucleus donor cells,(2) cytoplast of the goat MII oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species,(3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos.

由此结果得出以下结论:(1)山羊M II期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率;(2)山羊M II期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育;(3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的。

Although the ability to reprogramming of Kun Ming white mouse oocytes cytoplast is not effectively, reprogramming ability has improved when cystoplasts from oocytes and embryos work together.

昆明白小鼠卵母细胞胞质对体细胞核的重新编程能力较弱,而当其与正常受精卵的胞质共同作用时可较好地改善对体细胞的重新编程能力。

Leaf blade usually lanceolate to ovate, rarely linear or subulate, herbaceous, rarely leathery.

叶片通常披针形的到卵形,很少线形的或钻形,草质,很少革质。

Therefore, the oocytes quality decline induced by ooplasmic defect may be the main reason that cause older female sterility and pregnancy failure.

因此,卵胞质缺陷诱发的卵母细胞质量的下降可能是引起许多老龄妇女不育和怀孕失败的主要原因。

Ooplasmic transfer increases the fertilizability of the aged oocytes and the early embryonic viability, it has led to the birth of health babies, and become a hotspot in the realm of human assisted reproduction biotechnology and animal clone.

卵胞质转移技术能有效改善老龄卵母细胞的受精能力和早期胚胎的发育能力,在人类已有健康后代出生,它已成为人类辅助生殖生物技术和动物克隆研究的新热点。

Leaves simple; petiole 1.5--4(--7) mm; leaf blade ovate or lanceolate in outline, pinnatipartite or pinnatisect, sometimes pinnatilobed, 0.5--5 × 0.3--3 cm, leathery, both surfaces sparsely puberulous or subglabrous, base cuneate or subtruncate; lobes in 1--3(or 4) pairs, undivided and linear or subrhombic and unequally 2- or 3-lobulate, 4--15 mm, apex acute; lobules ovate, triangular, or linear; midvein abaxially slightly prominent or flat, inconspicuous.

单叶 叶柄1.5-4(-7)毫米;叶片卵形或披针形轮廓,羽状深裂或羽状全裂,有时羽状浅裂的, 0.5-5 * 0.3-3 厘米,革质,两面稀疏被微柔毛的或近无毛,基部楔形或近截形;在对,不裂和线形或1-3(或4)内裂片和不相等2或3具小裂片,4-15毫米,先端锐尖;卵形,三角形的小裂片,或线形;中脉的背面的稍突出或平,不显眼。

Fruit ovoid or subglobose, slightly compressed dorsally or laterally, glabrous; dorsal and intermediate ribs slightly thickened, corky, or somewhat protruding, filiform, subequal; lateral ribs dilated, subtriangular, corky; vittae solitary in each furrow, 2 on commissure.

果卵球形或近球形,稍压扁背或,无毛;稍加厚的背侧和中间棱,木栓质,或有点突出的,丝状,近等长;侧棱膨大,近三角形,木栓质;油管1在每棱槽,在合生面上2。

The plasmid pcDNA3.1-3-1E and pcDNA3.1 was respectively used to immunize SPF chicks by injection intra-muscularly in thigh muscle at 14 and 21 days of age.Chicks in each group except that in the unchallenged control group were challenged with 5×104 E.acervulina sporulated oocysts at 28 days post-inoculation,and the immune protective efficacy was observed.

将构建的重组质粒以及空载体质粒分别于14、21日龄分2次经腿部肌肉注射免疫SPF雏鸡,28日龄除非免疫非感染对照组外各组攻击性感染5×104个堆形艾美球虫孢子化卵囊,观察真核表达质粒对球虫感染鸡的免疫保护作用并探讨其免疫保护机理。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection,ICSI)授精的胚胎发育至6~8细胞期的1~2个单卵裂球,采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域,用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物,再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变,从而筛选出正常胚胎移植。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明:(1)水牛精子胞质内显微受精的雌、雄原核发育不同步,雌原核比雄原核早3h形成;(2)水牛卵母细胞ICSI和激活后用1.9mmol/L 6-DMAP培养处理3h能提高其胚胎发育率;(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率;(4)死精子能用于水牛卵母细胞的ICSI;(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

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随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。