卵质体

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

Comparisons were made with that of liposome made from soybean lecithin.

并与大豆卵磷脂脂质体的性质进行了比较。

objective to study the stability and degradation kinetics of amphotericin b in liposome.

目的 考察以卵磷脂、胆固醇为膜材制备的两性霉素b脂质体的稳定性及降解动力学。

Ingredients: natural moisturizing factor, lecithin, vitamin C, E, placenta nourishing and activating essence, ossein liposome, deep sea vegetal essence, bio whitening essence etc.

成分：天然水元保湿因子、卵磷脂，维他命C、E、胎盘营养活肤素、骨胶原脂质体、深海植物精华、生物美白素等。

The spore germination and gametophyte development of 21 ferns are studied. It indicates that :① the development period of prothallium and young sporophyte of the same species is different due to seeding time of spores;② the optimal temperatures of spores germination and gametophyte development of 21 ferns are about 15 -24 ℃;③ the rate of spore germination of rare ferns is much lower than the ferns which occur large populations in nature;④ the treatment with GA 3 can accelerate the germination of the spores;⑤ the prothallium changes from large to small, green to yellow when young sporophyte comes out. The prothalliums of all 21 ferns die away after the appear of the 3rd leaf of sporophytes;⑥ shapes of the 1st and 2nd leaves of the young sporophyte are different from those emerged later;⑦ spores cultured in dark can not germinate;⑧ a prothallium can develop only one young sporophyte though it has many archegoniums, and the spore propagation with leaf mould substrate is an economical method.

摘 要：以腐叶土为培养基质，对 21 种蕨类植物进行了孢子萌发和原叶体发育的研究，结果表明：①不同时期播种的同种蕨类的孢子，发育出原叶体和幼孢子体所历经的时间长短不同；②孢子萌发和配子体生长发育的适宜温度约为 15 ～ 24 ℃；③稀有蕨类的孢子萌发率低，而在野外能形成较大种群的蕨类的孢子萌发率高；④用 GA 3 处理孢子可以促进萌发；⑤当原叶体上长出幼孢子体时，原叶体由大变小，由绿变黄， 21 种蕨类的原叶体都在幼孢子体上长出第 3 片叶时消失；⑥幼孢子体上长出的第 1 、 2 片叶在形态上与以后长出的叶不同；⑦孢子萌发需要光；⑧ 1 片原叶体尽管有多个颈卵器，但仅发育出 1 株幼孢子体；⑨利用腐叶土进行蕨类孢子繁殖是一种经济实用的繁殖方法。

Microinjection and particle gun were used to transfer pCMV-β into fertilized zebra fish eggs. After staining analysis, the data showed80% and 6% expression rates, respectively.

此研究利用显微注射与粒子枪基因转殖法转殖pCMV-β质体进入斑马鱼授精卵，经染色分析得到80%与6%之表现率。

The results showed that : 1 During nonbreeding season, the giant panda′s ovary was still rich with large and small follicular oocytes, and the large follicular oocytes could be matured in vitro; 2 The mean diameter of the oocytes was (141±6.7)μm and lots of cortical granules contained in the cytoplasm of oo...

结果表明∶（1）在非繁殖季节，大熊猫卵巢中小腔及大腔卵泡卵母细胞仍较丰富，其大腔卵泡卵母细胞可在体外培养成熟；（2）大熊猫的卵母细胞平均直径为（141±6.7）μm ，其胞质富含卵黄颗粒；（3）自配 PandaI I号获能液（含丙酮酸钠、肾上腺素等）可使大熊猫精子有效获能；（4）大熊猫卵母细胞体外受精后，经培养可观察到第2 极体，有的受精卵开始发生卵裂。

The results show that the nano-magnetoliposomes prepared contain As2O3. They are spheric or elliptic particles with clear magneticcore.

结果所得纳米As2O3磁性脂质体粒径为(190±80)nm，球形或卵球形，有一个明显磁性核心，As2O3的包封率为53.0%。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下，卵母细胞外的卵丘细胞紧紧包裹卵母细胞，且与卵母细胞之间有大量细长的微绒毛相联系；卵丘细胞核呈卵圆形，其长径为4250±0.042μm，短径为2.750±0.071μm；透明带均匀，与卵母细胞结合紧密；在皮质区集中分布大量的多峰型线粗体、脂滴和空泡，线拉体长径为0.600±0.106μm，短径为0.490±0.117μm；细胞核中颗粒状的核质分布非常均匀。

Most sperm nuclei eventually developed into the male pronuclei, just like the normal sperms, except sperm nuclei in a few zygotes kept dense throughout the fertilization process. Dense chromosome body was seen at the nuclear area during the prophase of first mitosis, and in the middle of spindle at metaphase of first mitosis.

精子入卵后形成精核，大多能够逐渐解凝、液化并膨大，最终形成形态正常的雄性原核，但在第一次卵裂早期退化成浓缩的染色质小体，并在胞质分裂时随机地分配到其中一个子细胞里的分裂沟附近。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。