卵质

In the present, we reviewed the data about the structure and what function of this protein in the ER and gamete fusion.

就目前关于PDIA3的结构和功能及在精卵质膜融合中的作用研究进展做一概述。

There was a first polar body which was still connected with the egg near the fertilizing sperm.

许多学者都证实了精子通过卵膜孔首先与该孔下面卵质膜一个特殊区域称为精子穿人部(印erm entry site)发生结合和融合，然后并人卵内。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物，从猪卵巢上获得的卵母细胞数量虽然较多，但成熟度比较低；由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内，跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应；如果卵胞质成熟或活化程度不够，加之圆形未成熟精细胞成熟度比较差等原因，很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足，引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等，使ROSI显微受精失败。

The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

应用该技术对猪、山羊和牛不同大小、不同健康程度卵泡及体内和体外成熟过程中卵母细胞染色质构型的变化发现：1）不同动物卵母细胞染色质构型不同；猪和牛卵母细胞染色质围绕核仁凝集形成环，但山羊卵则没有此环。2）卵母细胞在GVBD之前都同步在某一染色质构型；猪同步在GV1，山羊GV3n，牛GVf，说明这些染色质构型代表着进行性变化。3）染色质构型的变化反映基因转录活动的变化。4）染色质构型受环境变化的影响。

Observation on the year-round spawning and the discrimination of larval activity of Epinephelus coioides in captivity were carried out in the laboratory.

对5~7龄斜带石斑鱼亲鱼的周年产卵、产卵量、卵质以及初孵仔鱼活力的判别进行了观察研究。

This paper reviews the ooplasmic transfer in summary, the relation between ooplasm and fertilization and embryo development, the hereditary behavior of heterogenous mitochondria DNA and it's examination after ooplasmic transfer.

通过卵胞质转移研究概况、卵胞质与受精和胚胎发育、异种线粒体DNA遗传方式和卵胞质转移遗传物质的检测4个方面对卵胞质转移技术进行讨论。

Including the soybean lecithin separation purification, gamma - the linolenic acid separation and the purification, as well as gamma - linolenic acid fat 质体 Bao He studies and so on, and carries on optimal using the orthogonal experiment to the above craft.

其中包括大豆卵磷脂的分离纯化，γ-亚麻酸的分离和纯化、以及γ-亚麻酸脂质体包合研究等等，并利用正交试验对上述工艺进行优选。

East valuable Jin Danzhong the gamma - linolenic acid and the soybean lecithin will make the fat 质体 both to conform to the original group side principle and to be possible to increase the preparation the stability and the curative effect.

将东宝金丹中γ-亚麻酸和大豆卵磷脂制成脂质体既符合原组方原则又可增加制剂的稳定性和疗效。

However, crucian carp in Honghu Lake showed a tendency to produce relatively larger eggs than in Dongting Lake, based on measurements of the egg diameter in the ovary at stage Ⅳ.

但Ⅳ期卵巢最大卵径的测量结果表明，洪湖鲫繁殖力下降并不伴随卵质下降，反而显示出卵子质量高于洞庭湖鲫的倾向。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。