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卵裂细胞

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Ontogenesis begins from the fertilized embryo. After the cells proliferation during the embryo cleavage, it enters the stage of blastoderm differentiation、 tissue and organ formation.

个体发育从受精卵开始,经过卵裂阶段的细胞增殖后,进入到胚层分化、组织和器官形成阶段。

FISH technique could be performed for the detection of HBV in human blastomere cells,and it has great significance to reduce vertical transmission of HBV in IVF-ET.

荧光原位杂交技术可用于卵裂球细胞HBV感染的检测,在体外受精-胚胎移植实践中对降低HBV垂直传播有重要意义。

The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.

2-细胞胚胎卵裂球随机分布在囊胚的胚胎部分和胚外部分。

One embryonic cell can be separated into many blastomere cells. As be the nuclear donor of the embryonic cell nuclear transfer, it can breed the number of eminent species largely, increase the number of endangered animals, supply large number of supply same genotype animals, improve the efficiency of gene transfer and sex control and offer same genotype apparatus or organ for human transplant.

一个早期胚胎细胞可以分离多个卵裂球,以此作为核供体进行胚胎细胞核移植,可以大量扩繁优良品种的数量,增加濒危动物的数量,为科学研究提供大量遗传同质的动物,提高转基因和性别控制技术的效率,提供基因型相同的器官或组织供人类移植。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域,用限制酶 Bfm 消化 PCR产物,10 %聚丙烯酰胺凝胶电泳检测。

Porcine was acted as the experimental animal. It was researched on problem of the origin of obtaining early embryonic cells, methods of separating blastomere cells, best parameter and order of ECNT embryo electric activation and system of ECNT embryo IVC, which was to find a scientific method of fitting ECNT and a procedure of fitting the lab producing embryos largely, optimize the procedure of ECNT and improve the ECNT embryo in vitro.

本实验以猪为实验动物,对猪胚胎细胞核移植供体的来源、卵裂球细胞分离方法、核移植重建胚的电激活最佳参数、核移植受体的激活时序以及核移植重建胚体外培养系统相关问题进行了研究,旨在探索一种适用于猪胚胎细胞核移植的科学方法,提高猪胚胎细胞核移植胚胎的体外发育率,优化胚胎细胞核移植程序。

Interestingly, a monopolar spindle was assembled in each blastomere in the second cell cycle, and disjunction of duplicated chromosomes and the cleaage was preented, leading to the chromosome set doubling.

有趣的是,在第二个细胞周期中,每一个分裂球中都组装了一个单极纺锤体,同源染色体分离和卵裂被阻止,导致染色体组加倍。

It transforms the zygote into a cluster of cells called morula which, seen through a microscope, resembles a mulberry.

卵裂把受精卵转换成称为桑葚胚的细胞团,它在显微镜下看起来象桑葚果。

There were no significant difference amongedifferent adult skin fibroblasts on the cleavage and development of reconstituted embryos.

研究发现来自不同个体的皮肤成纤维细胞所构建的重组胚,卵裂率和囊胚发育率无差异。

Tetraploid veliger were carried out with four chemicals on this scallop in 1998—2000. Tetraploid were induced by inhibition of the polar body and first mitosis with cytochalasin B, colchicine, 6-DMAP and caffeine combined with CB, respectively.

中文摘要:1998—2000年采用细胞松弛素B、秋水仙素、6-二甲基氨基嘌呤(6-DMAP)以及咖啡因等药物抑制虾夷扇贝第一极体(PB1)释放、PB1和第二极体(PB2)释放以及抑制第一次卵裂等方法,诱导虾夷扇贝四倍体。

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