卵裂的

It showed that the cumulus cells did play significant role in the stimulation of nuclear maturation of porcine oocytes and subsequent development.

表明卵丘细胞对猪卵母细胞的核成熟及卵母细胞体外受精后的卵裂和早期胚胎发育，都具有明显的促进作用。

The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

试验获得了如下结果：1。以山羊耳成纤维细胞为供体，以牛、羊、兔卵母细胞做为胞质受体构建重构胚，并经体外培养，发现不同的胞质受体均能支持重构胚的早期发育，但受体胞质对重构卵的重构胚早期发育速度和卵裂率均有显著影响。

Stigma 2-cleft. Fruit indehiscent, small, obovoid to globose, included or nearly included in calyx, 1-(or 2)-seeded.

柱头2半裂。果不裂，小，倒卵球形的到球形，包括或差不多内藏于花萼，1-(或2)种子。

Capsules baccate, ovoid or subglobose, 2-2.5 × 1.5-2 cm, red-yellow to purple when mature, indehiscent, or 3-valved when dry; arils white.

蒴果浆果状，卵形体或近球形， 2-2.5 * 1.5-2 厘米，成熟时红色黄到紫色，不裂，或干燥时3瓣裂的；假种皮白色。

The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 ms, two) to reconstruct bovine cloned embryos.

以核移植胚胎的卵裂率、囊胚发育率作为检测指标，对不同的方法所获得的克隆胚胎的卵分裂率与囊胚发育率进行比较，最后筛选获得1个优化的牛体细胞核移植操作程序，即采用Spindle view系统对牛卵母细胞进行去核操作，将供核体细胞注射到卵周隙，然后通过电融合法将供体核引入去核卵细胞质(电融合参数为1.9 kV/cm，脉冲时程10 ms，方波2次间隔2 s)。

Analysis of mitochondrial DNA composition of the panda-rabit cloned embryos found that mitochondria from both panda somatic cells and rabbit ooplasm co-existed in early blastocyst, but mitochondria from rabbit ooplasm decreased and those from panda donor cells dominated in early fetuses after implantation.

结果显示，应用卵裂球电融合方法可以制作一系列的多倍体胚胎；小鼠囊胚的形成与胚胎中的细胞数目无直接关系，卵裂球的电融合和染色体数目的增加不会改变胚胎的发育进程；而胚胎细胞中的核／质比可能是控制胚胎囊胚化的一个重要因素。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

5 Mm. Female flowers: pedicels short but extending to ca. 5 mm in fruit; sepals 5, oblong-ovate to ovate-lanceolate, ca. 8 mm; petals and disk as in male; ovary ovoid; styles 3, apex bifid.

雌花：花梗短，但在果期延伸到约5毫米；萼片5，长圆状卵形的到卵状披针形，约8毫米；如同在男性里的花瓣和花盘；子房卵球形；花柱3，先端2裂。

Methods and Results Experiment 1: Intracytoplasmic injection of rat sperm heads was performed using 7~10 μm and 2~4 μm diameter pipettes and different injected method respectively.

方法和结果实验1：用直径为7~10μm和2~4μm的注射针以及相应的注射方法进行大鼠ICSI，ICSI后卵母细胞存活率(30.5% vs。 61.3%)和卵裂率(12.5% vs。 51.1%)均差异显著(P.05)；实验2：分别在hCG后14 h、16 h和18 h取卵进行ICSI，三组存活率77.4%、74.1% vs。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。