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卵裂

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It transforms the zygote into a cluster of cells called morula which, seen through a microscope, resembles a mulberry.

卵裂把受精卵转换成称为桑葚胚的细胞团,它在显微镜下看起来象桑葚果。

At water temperature of 16.0-17.2 ℃, the process of embryonic development could be divided into 24 stages, which were described as follow: fertilized egg at 0 min, 2-cell stage at 75 min post fertilization; myotome forming stage at 43 h PF, and hatching-out stage at 94 h PF when the accumulative temperature reached 1616.8 ℃.h, and the average size of larvae was about 3.8 mm in body length.

梭鲈胚胎发育过程可分为24期,在水温16.0~17.2℃范围内,受精75 min开始第1次卵裂,受精后43 h开始形成器官,受精后约94 h开始出膜;刚出膜的仔鱼全长平均为3.8 mm;从受精到孵化出膜总积温为1 616.8℃。h。

After extrusion of polar body and the completion of the first cell cycle, microfilaments concentrated to the cleavage furrows.

极体排出和第一次有丝分裂时微丝在卵裂沟处富集。

Objective To investigate the relationship between the first polar body morphology of oocyte and the zygote morphology in pronucleus stage, embryo quality in cleavage stage.

目的 探讨卵细胞第一极体形态与原核期受精卵形态、卵裂期胚胎质量的关系。

The results show that FISH can be used in experimental study and clinical application of PGD; 3. The cause of multi- pronucleus in embryos from IVF or ICSI would be different.

IVF受精和 ICSI受精的3PN胚胎发育有差异,证明*F受精和 ICSI受精的3PN中的原核的来源是不同,导致胚胎第一次卵裂的方式也不同。

There were no significant difference amongedifferent adult skin fibroblasts on the cleavage and development of reconstituted embryos.

研究发现来自不同个体的皮肤成纤维细胞所构建的重组胚,卵裂率和囊胚发育率无差异。

The optimization of heat shock conditions for producing tetraploidy loach was treated the fertilized eggs for 4 min with 41℃ water after fertilization about 20 minutes.

采用热休克抑制受精卵第一次卵裂、Ag-NOR计数鉴定四倍体胚胎的方法,探索了诱发四倍体泥鳅的最佳条件是:在受精后20 min左右采用41℃热水持续处理泥鳅受精卵4 min。

It indicated that the yield of tetraploidy not more than 25% induced by inhibiting the first mitosis with CB, colchicine and 6-DMAP, respectively, while it can be induced with higher percentage by blocking PB1releasing with CB. The tetraploidy percentage of groups shock began at 15—22min after fertilization are higher than that shock began at 42min, and the yield of tetraploidy also increased with the CB density and time lasting.

结果表明,CB、6-DMAP和秋水仙素抑制扇贝第一次卵裂诱发四倍体的比例低于25%;采用CB抑制PB1可有效地诱导产生四倍体,从授精后42min提前到15—22min开始处理,抑制PB1的放出有助于提高四倍体的比例,在12℃,处理开始和终止时间分别在授精后20—22min和62—67min时(即PB2始出现时),面盘幼虫四倍体率最高,为56.5%。

The development speed of gynogenetic embryos was later than that of the control group, and aneuploids were found in this study. The obvious improved survival of trochophore larvae with a peak at 40s UV exposure suggested the presence of a Hertwig effect in the gynogenesis of A. pectinata. When sperms were irradiated for 40s, the cleavage rate was relatively high (51.2%), the developmental rate of D-larvae became zero, and the rate of haploid reached the highest (36.8%).

经紫外线照射的精子受精后所产生的单倍体胚胎发育速度慢于正常受精所产生的二倍体胚胎,各照射组均出现非整倍体。40s照射组中卵裂率达到51.2%,D型幼虫发生率为0,单倍体率最高,达到36.8%,出现"Hertwig效应"。

Tetraploid veliger were carried out with four chemicals on this scallop in 1998—2000. Tetraploid were induced by inhibition of the polar body and first mitosis with cytochalasin B, colchicine, 6-DMAP and caffeine combined with CB, respectively.

中文摘要:1998—2000年采用细胞松弛素B、秋水仙素、6-二甲基氨基嘌呤(6-DMAP)以及咖啡因等药物抑制虾夷扇贝第一极体(PB1)释放、PB1和第二极体(PB2)释放以及抑制第一次卵裂等方法,诱导虾夷扇贝四倍体。

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