卵裂

Single blastomere PCR is stable and reliable,and it can be used for preimplantation genetic diagnosis.

目的建立单卵裂球PCR技术，为开展单基因病的着床前遗传学诊断奠定基础。

FISH technique could be performed for the detection of HBV in human blastomere cells,and it has great significance to reduce vertical transmission of HBV in IVF-ET.

荧光原位杂交技术可用于卵裂球细胞HBV感染的检测，在体外受精-胚胎移植实践中对降低HBV垂直传播有重要意义。

The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.

2-细胞胚胎卵裂球随机分布在囊胚的胚胎部分和胚外部分。

Blastomere numbers and morphology scores were highest in group Ⅰ(P.05), but no significant differences existed between group Ⅱ and group Ⅲ.

组的胚胎卵裂球数目及胚胎形态评分均显著优于Ⅱ组及Ⅲ组(P.05)；Ⅱ组与Ⅲ组相比差异无统计学意义。

Hence, potentially viable embryos will be discarded upon screening a single blastomere.

因此，有可能对单个卵裂球的基因筛选导致一个有活力的胚胎被丢弃。

The repeated detection of single blastomere of untransferred embryos verified the PGD results, and further indicated that our PGD technique of ACH was relatively stable and reliable.

通过对未移植胚胎的单卵裂球的重复检测，验证了PGD的结果，进一步说明了我们开发的ACH的PGD技术是较稳定、可靠的。

F508 mutation detection on the single blastomere was performed with nested polymerase chain reaction.

同时利用套式聚合酶链反应技术，对活检所得的单个卵裂球分别进行ΔF508 突变基因检测。

BACKGROUND: Presently, the method of establishing human embryonic stem cells mainly contains blastula method, nuclear transplantation method and embryo blastomere method.

背景：目前人胚胎干细胞建系的技术路线主要有囊胚法、核移植法和胚胎单卵裂球法，但由于破坏胚胎，涉及诸多伦理问题。

We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我们采用了一种利用单细胞标本通过二重巢式PCR并结合ARMS技术确定中国人群中广泛的β-地中海贫血突变谱的基因型的策略，通过对含有β-珠蛋白基因CD41-42突变的携带者5个该基因常见突变位点基因型的确定，以及正常人的单个淋巴细胞、单卵裂球的β-珠蛋白基因该5个位点基因型的确定，对这种技术条件的优化、准确性和可靠性进行了研究。

The average amplification efficiency of single blastomere was 89.7％ and the average allele drop-out rate was 14.4％.

单个卵裂球平均扩增效率为89.7％，等位基因脱扣(allele drop-out， ADO)率为14.4％。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。