卵膜

But when the cholesterol was substituted by its oxide result, the membrane life time extended to nearly 60 hrs ;besides the diameter of the hole on which the membrane exists 、temperature、PH value cause great effects to the life time of the membrane, and the diameter was the most important element. When the diameter larger than 3mm it is almost no way to form a membrane on the hole under experiment condition. Between 1mm and 3mm it has a better result by change the prescription of the membrane. when it is 0.5mm all the membrane can last more than 40 hrs ,and the prescription like Och : phosphatidylcholine / cephalin＝4:1; 3:1; 2:1; 1:1; 2:3 need the request of the experiment basically, when cooperate with multi hole structure it is very to detect the concentration and found the model.

研究结果说明，成膜在普通胆固醇/磷脂配方下不能达到满意的结果，无论如何改变外界条件成膜始终超不过7小时，而将胆固醇换成氧化胆固醇再和磷脂按照一定配比的时候，成膜时间则大大延长，可达60h左右；另外除成膜配方，成膜孔径、温度、PH值等因素对成膜有着很重要的影响，成膜孔径对膜的影响最为关键，在3mm以上的孔径时成膜比较困难，在1－3mm适当提高成膜液浓度可以改善，孔径为0.5mm左右成膜比较稳定基本都能达到40h以上，合适的几个配方如卵/脑磷脂：氧化胆固醇＝4：1；3：1；2：1；1：1；2：3基本满足实验要求，配合一板多孔结构，可以保证单位时间的离子传输量的检测和计算。

In ovary, PNA receptors appeared in the oocyte cytoplasm of second phases of oogenesis; the positive granules gradually increased from third to forth phases, and they exhibit a maximum expression before vitellogennic stage in cytoplasm of oocyte; from vitellogennic stage to chorionation stage, the positive granules gradually reduced. Binding sites on follicle cells were changed with their morphological variation in every stage of oogenesis.The staged and specific expression of oncogene c-kit, the tyrosine-kinase receptor, is closely related with gametogenesis.

2在中华蚱蜢卵子发生的早期，PNA受体是由卵母细胞自身合成；在卵黄发生前的准备时期，卵母细胞质中的PNA受体一部分来源于自身合成，另一部分则来源于滤泡细胞，而糖复合物的大量出现可能直接与卵黄发生有关；卵黄发生期PNA受体的减少可能是在卵黄物质形成中部分发生了修饰；卵黄和卵壳的相继形成过程中，阳性反应的出现说明PNA受体参与了卵黄膜及卵壳的形成，而这些受体物质是由滤泡细胞分泌的。

According to the size and shape of oocyte, the morphology of nucleolus, the growth of yolk and the structure of follicle, oogenesis of H. d. supertexta can be divided into three stages as follows: oogonium, previtellogenic oocyte and vitellogenic oocyte. The ovary wall is composed of tunica adventitia and germinal epithelium which will produce oogonia and follicle cells. The follicle is the structure unit of ovary.

根据卵细胞的大小、形状，核仁的形态，卵黄颗粒的积累情况，滤泡的结构等，将九孔鲍卵子的发生分为卵原细胞、卵黄发生前的卵母细胞和卵黄发生期的卵母细胞3个时期；卵巢壁由外膜及内生殖上皮构成，生殖上皮分化产生卵原细胞和滤泡细胞；卵巢的结构单位是滤泡。

The polar body extrusion process of induced maturation is similar to naturally fertilized eggs.

在卵母细胞成熟过程中有受精膜举起的现象，说明精子入卵不是受精膜举起的必要条件。

Several other diagnostic procedures including intradermal test, circumoval precipitin test and cercarienhullen reaction were also employed at the same time for the 67 proved cases, and their positive rates were found to be 41.7%, 80.6-94.0% 48 or 72 hours' i...

粪检阳性组的尾蚴膜和环卵反应阳性率分别达97.0％和94.0％。治疗后复查表明，环卵反应转阴率较尾蚴膜反应为高。作者还讨论了用日本血吸虫各期抗原检查曼氏血吸虫病病人及考核疗效的价值。

Spikelets laterally compressed, florets several, all bisexual or lowest sterile and without a palea; rachilla glabrous, disarticulating above glumes and sterile floret and between fertile florets; glumes lanceolate, shorter than lemmas, subequal or unequal, scarious-membranous, 1–3-veined, obtuse to acuminate or aristulate; lemmas ovate-lanceolate, scarious-membranous, 3-veined, long-ciliate on lateral veins, keeled, apex setaceously bidentate with a short, often recurved awn from the sinus; palea shorter than lemma, hyaline, keels very shortly ciliate.

小穗侧面压扁，小花数个，全部不育和没有一内稃的最低的两性的或；高于颖片和不结果的小花无毛，脱节的小穗轴和在肥沃的小花之间；披针形的颖片，短于外稃，近等长或不等长，干膜质膜质，1-3脉，具小芒的钝到渐尖或；卵状披针形，干膜质的外稃膜质，3脉，具长缘毛在侧脉上，龙骨状，先端setaceously具双齿具短，通常下弯从那里那些凹缺；内稃短于外稃，透明，龙骨非常短的纤毛。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来，在基金委的支持下，我们以蛋白膜伏安法这一新技术作为主要的研究手段，并结合其他生物、化学技术，研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为，结合各种光谱技术，对蛋白质在膜中的构象进行表征，探索其电子传递机理。

It shows that the average thickness of the surface layer of crystals,the palisade and cone layer and the eggshell membrane are 20.8,220.8 and 62.5μm,respectively,which accounting for 6.8%,72.6% and 20.6% of the total thickness of the eggshell.There are many vesicular holes in palisade layer with an average diameter of(0.32±0.08)μm(n=30).The function of such holes may be of significant to air exchange.The shape of eggshell pore on the surface layer of crystals is round or ellipsis.The fracture surface of the pore is funnel-shaped.

结果表明：白冠长尾雉卵壳的表面晶体层、栅栏锥体层和壳膜层的厚度分别为20.8，220.8和62.5μm，分别占卵壳总厚度的6.8%，72.6%和20.6%；栅栏层有很多蜂窝小孔，其直径为(0.32±0.08)μm(n=30)；卵壳表面有许多圆形或椭圆形的气孔，可能与胚胎发育过程中的气体交换有关，气孔横断面呈细漏斗状，在其上半部有颗粒状填充物。

The results showed that the insulin-induced oocyte maturation was accompanied by the decreases in membrane potential, K+ transference number and intracellular K+ and H+ activities, and the increases in intracellular Na+ and Cl- activities.

Malle：等(198们的实验也证实了胰岛素及其类似物的这种作用。Lessman等〔1984)曾报道，胰岛素在促进卵母细胞成熟同时，膜的某些特性发生了变化，如膜阻抗增加，膜电容减少和膜电位降低去极化〕等。

METHODS Xenopus leavis females were injected with HCG, eggs were harvested next morning and dejellied by 2% cysteine, from which the Xenopus leavis egg cell free translating system was prepared.

而基因功能的研究离不开基因的表达，这就迫切需要一种操作简便，适用范围广的表达工具来表达有功能的蛋白质。方法非洲爪蟾注射HCG催卵，2%半胱氨酸去胶膜，高速离心制备卵提取物，即为非洲爪蟾卵非细胞翻译体系。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。