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Methods: Human mature oocytes were cryopreserved using cryoleaf. Resuscitated oocytes were administered by intracytoplasmic sperm injection, embryo transplantation or cultured to blastulation, observing the fertilization and developmental capacity of oocytes.

运用cryoleaf进行人类成熟卵母细胞玻璃化冷冻保存,复苏后行胞浆内单精子注射-胚胎移植或培养至囊胚形成,观察复苏后卵母细胞受精及发育能力。

1, cumulus cell had important role on nuclear and cytoplasmic maturation of oocytes.

1,卵丘细胞对卵母细胞的核和胞浆成熟均有重要作用。2。

objective the effects of anthocyanin pigment from maize purple plant on resisting lipid peroxidation were investigated.methods the inhibition of anthocanin pigment from maize purple plant was examined in vitro that autcoxudation of lecithin liposome system induced by fe2+.50 mice were randomly divided into 5 groups.vehicle and different dose anthocyanin pigment from maize purple plant were given respectively,and then experimental injury of mice liver was induced to make use of bromobenzene and the content of mda was determined in liver homogenate.results the inhibition rates of anthocyanin pigment from maize purple plant on linoleic acid oxidation was higher that of ascorbic ascorbic acids.the content of mda in homogenate in middle and high dose of pigment were significantly lower than that in low dose and the injured control group.there were no significat differences in the content of mda in homogenate between low dose group and injury control group.conclusion the anthocyanin pigment from maize purple plant have the capability to resist lipid peroxide.

目的 探讨玉米紫色植株色素抗脂质过氧化的作用。方法体外实验测定玉米紫色植株花色苷色素在fe2+引发的卵磷脂脂质体体系中抗氧化活性。体内实验,取50只小鼠随机分成5组,分别给予溶媒和不同剂量的色素,然后采用溴代苯致实验性肝损伤,测定肝匀浆的丙二醛含量。结果在由fe2+引发的卵磷脂脂质体体系中玉米紫色植株色素对脂质过氧化有明显的抑制作用,抑制率随样品的浓度增高而增大,并且明显优于抗坏血酸。在溴代苯致小鼠实验性肝损伤模型实验中,中、高剂量组的丙二醛含量均低于损伤模型组。低剂量组和损伤模型组比较丙二醛含量差异无统计学意义。

After that , the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article , in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos .

和胞浆内单精子注射两种方法对玻璃化冷冻卵母细胞的受精能力进行了比较研究,旨在建立能够进一步提高牛冷冻卵母细胞受精能力的改进方案,使卵母细胞冷冻技术上升到与胚胎冷冻同样成熟的水平。

Their shapes were diversiform such as nummular, elliptic and oval etc. Positive neurons had one or several tubers and its axon was short. This experimentation results showed that development level of eNOS immunopositive neurons approach the adult rabbit when it is a young rabbit.

eNOS阳性神经元呈棕褐色,免疫阳性物质主要位于胞浆内,胞浆染色均匀,胞核处着色浅;神经元胞体形态各异,有圆形、卵圆形、椭圆等多种形状,神经元突起有一个或者数个,轴突较短。

Results The expression of Foxo3a, Bim, FasL in oocyte nests and primordial follicles were detected in the nuclei and cytoplasms of some oocytes. The patterns of Bim and FasL positive rates were consistent with that of Foxo3a and TUNEL positive oocytes rates, which reached the peak in the ovaries of 2-day-old rats.

结果 免疫组化显示Bim和FasL都在新生大鼠部分卵母细胞巢和原始卵泡的卵母细胞核内和胞浆高表达,且阳性率曲线都在2d达到高峰,随后逐渐降低,与TUNEL和FoXo3a的阳性率变化曲线一致。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论:成骨细胞呈梭形,多突起;细胞核呈卵圆形,偏于一侧;胞体大,胞浆丰富;碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒,某些部位染成黑色,定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞;细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

These zygotes were divided into four groups according to the different zygote pronuclei orientation and the developmental potential.

根据早期卵裂时间及原核构型不同进行筛选而移植的129个体外受精、卵胞浆内单精子注射周期,共1 032个正常受精卵为资料。

In some insects with meriostic ovarioles, maternal germ plasm is assembled during oogenesis and contains germline determinants that originated from the perinuclear nuage in the nurse cells.

在某些具有营养细胞型微卵管的昆虫当中,母系生殖浆在卵发育的过程形成,而且生殖浆内所含生殖细胞决定物质系来自於营养细胞的生殖云。

This study compared outcomes of in vitro maturation and in vitro fertilization intracytoplasmic sperm injection cycles after IVM of immature germinal vesicle oocytes.

研究对比体外受精-单精子卵胞浆内注射周期所获生殖泡期不成熟卵母细胞以及体外成熟周期所获GV期卵母细胞体外培养成熟后的胚胎发育情况。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。