卵泡的

Objective To study the differentially expressed proteins between mature and antral follicles and identify the proteins crucial for follicle development and oocyte fertilization.

目的 应用蛋白质芯片和质谱技术，建立人成熟卵泡液及窦卵泡液的蛋白质表达指纹图谱，寻找在卵泡生长发育过程中起关键作用的蛋白质。

Immune POF model elicited by immunization in subcutaneous with antigen sourced isotype animal ovaries can cause growing follicles and mature follicles decrease obviously, the morphology of oocytes and zona pellucida abnormal, the adjacent granular cells disorder and atresic follicle increase.

以同种动物卵巢为抗原主动免疫建立的免疫性POF模型可致生长卵泡和成熟卵泡数明显减少，卵母细胞及透明带形态异常，周边颗粒细胞排列紊乱，闭锁卵泡增多，病理损伤随免疫时间延长而加重。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

The vast majority of follicles undergo a degenerative process in reproductive life. Apoptotic cell death is the fundamental mechanism in response to follicular atresia. Moreover, the apoptosis of granulose cells is implicated in directly mediating atretic process. Multiple studies have confirmed that the apoptotic process of GCs is not only related to apoptotic genes, but triggered by cytokines related to reproduction and Caspases family.

中文题名几种生殖激素对体外培养的牦牛卵泡颗粒细胞凋亡的影响副题名外文题名 Effects of several reproductive hormones on apoptosis of ovarian granulose cells of yaks in vitro 论文作者杨世华导师余四九教授学科专业临床兽医学研究领域\研究方向学位级别博士学位授予单位甘肃农业大学学位授予日期2002 论文页码总数108页关键词牛类固醇激素分泌卵泡颗粒细胞培养牦牛生殖激素馆藏号BSLW /2003 /S823 /1 卵泡闭锁是哺乳动物卵巢中极为普遍的自然现象。

FSH is one of the most important endocrine factors in regulating follicular development.

卵泡刺激素是调节卵泡发育重要的内分泌激素，同时许多卵巢内局部产生的生长因子对卵泡发育也有重要的调节作用。

Cell adhesion and extra cellular matrix in follicles also play an important intraovarian role.

卵泡闭锁的实质就是卵泡中发生了细胞凋亡，其中卵泡颗粒细胞凋亡是导致卵泡闭锁的直接诱因。

The level of oestradiol (E2) arrived the highest value (48.24pg/mL) in the sixth day of culture for follicles with a diameter of 100m above in the 10% BFF group.

表明培养液中添加一定比例牛卵泡液可显著刺激体外培养腔前卵泡E2的分泌，促性腺激素存在下更加强了卵泡液对E2分泌的刺激作用。

Results: The Ang II levels increased significantly in the atresia follicles, either in follicular fluid or in the medium of cultured thecal cells. At the same time the renin activity levels decreased markedly. No significant change in the levels of both Ang II and renin activity was found in the medium of cultured granulosa cells.

结果：卵泡在闭锁状态下的Agn II水平在卵泡液中以及卵泡膜细胞培养液中均显著升高，肾素活性水平明显下降；颗粒细胞培养液中两项指标均无明显改变。

The size of follicle begin primordial follicle to tertiary follicle(1.5～3mm).The number of mitochondrion increased,and the cristae increased.Gogi body change to type,cortical granule increased,and moved behind cytolemma.The proportion of small,middle and large follicles on ovary were 92.51%(92.51±5.87),4.60%(4.60±4.91) and 2.88%(2.88±2.22) respectively.

卵泡在发育过程中，卵泡细胞形成的突起与卵母细胞始终保持密切的联系：卵母细胞从原始卵泡到直径1.5～3mm的三级卵泡，细胞质中线粒体数量由少到多，线粒体内的嵴不断增加，高尔基体由不典型到变成典型，皮质颗粒由开始形成到形成较多的皮质颗粒，并逐渐地迁移到卵母细胞膜下。

The clue is the prominent eosinophilic and hyalinized so-called "glassy membrane" band in between dark spindle granulosa layer and lutinized theca interna. There are scattered lutinized thecal cells in theca interna with ample pink cytoplasm which are usually seen in childhood or pregnancy.

诊断线索是明显的嗜酸性胞质和位于暗梭形颗粒细胞层和黄素化卵泡膜之间的透明化的所谓&透明膜&带，在卵泡膜内层有散在黄素化卵泡膜细胞，胞质淡粉染，这种细胞常见于儿童期或孕期。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。