卵泡的

The follicular development periods of the Zaocys dhumnades were divided into four stages: oogonium, primary follicle, growing follicle and mature follicle, and the growing follicle was the important phase in the follicular development periods.

乌梢蛇卵泡的生长发育可以分为卵原细胞、初级卵泡、生长卵泡和成熟卵泡等4个时期，其中生长卵泡是卵泡生长发育的重要阶段。

We detected that EGF mRNA was expressed sflungly lii the oocyte, and is also found hi gmnulosa cells, the cell fium smaller foflicular expressed stronger than fium bigger one. In the corpus hemonbaglcwn corpus luteurn, lean type and pseudocorpus-luteum, EGF rnRNA was detected,, no distinct difference can be seen in them. The EGF mRNA expressed strongly in fimbria end, ampulla and isthmus of oviduct, in the big follicular stage, ovulation stage, pregnancy stage and spurius pregnancy stage, we can not see any distinct change in them, but hi the medium follicuar stage,it is weaker.

结果发现：猪卵母细胞中EGF的mRNA强烈表达，且小卵泡卵母细胞→中卵泡卵母细胞→大卵泡卵母细胞中，EGF的mRNA表达量有逐渐减少的趋势；猪卵泡的颗粒细胞中有EGF的mRNA表达，小卵泡颗粒细胞→中卵泡颗粒细胞→大卵泡颗粒细胞中，EGF的mRNA表达也有逐渐减少的趋势；猪卵巢中的红体、黄体、白体和假黄体中都有EGF的mRNA表达，看不出几部分的表达量有明显的强弱变化；猪输卵管伞部、壶腹部和峡部，都有EGF的mRNA表达，在大卵泡期，排卵期，孕期和假孕期都强烈表达，各期间看不出明显的强弱变化，中卵泡期表达较弱；猪子宫中EGF的mRNA在大卵泡期，排卵期，孕期和假孕期都强烈表达，看不出表达量的明显变化，而小卵泡期表达量明显减弱。

This procedure can be influenced by a lot of factors, such as hormones, growth factors, et al. Only very few follicles will develop into dominant follicles and ovulation and more than 99% turn atresic. Xiang pigs are a world famous breed of the miniature pig mainly distributed in Guizhou and Guangxi provinces of China.

同时，卵泡的生长和发育也是一个高度协调的生理过程，受多种因素（激素、生长因子、芳香化、金属离子、物理及化学刺激等）的影响和控制，少数卵泡最终发育为优势卵泡并排卵，99%以上的卵泡都将发生闭锁。

This procedure can be influenced by a lot of factors, such as hormones, growth factors, et al. Only very few follicles will develop into dominant follicles and ovulation and more than 99% turn atresic. Xiang pigs are a world famous breed of the miniature pig mainly distributed in Guizhou and Guangxi provinces of China.

同时，卵泡的生长和发育也是一个高度协调的生理过程，受多种因素（激素、生长因子、芳香化酶、金属离子、物理及化学刺激等）的影响和控制，少数卵泡最终发育为优势卵泡并排卵，99%以上的卵泡都将发生闭锁。

E. staining and other techniques were used to elucidate the pattern of follicular development, atresia, changes in follicle number on the ovarian surface, proportions of apoptotic granulosa cells in follicles of different sizes and morphological changes of atretic follicles, as well as changes in serum estradiel and progesterone levels at different stages of the porcine estrous cycle.

为探讨猪发情周期不同时期卵泡发育和闭锁的规律及机理，本实验通过TUNEL原位标记、H.E。染色以及放射免疫测定等手段研究了猪发情周期不同阶段卵巢表面各类卵泡数量的变化、各类卵泡中颗粒细胞的凋亡比例、闭锁卵泡的形态变化以及发情周期各阶段血清孕酮及雌二醇水平的变化等问题。

The BMP15 protein played a certain role in all the stages of follicular development and had a relationship with the luteal dissolution mechanism; In the atretic follicle, the low expression of BMP15 protein maybe caused by inhibition from the other factors which lead to connected modification of follicular atresia.

结论］BMP15参与了兔卵泡发育全过程，并且与黄体溶解机制有关；而在闭锁卵泡中，其低水平的表达可能是由其他因子所抑制，进而共同调节了卵泡的闭锁。

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF。

Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3-6mm卵泡的卵泡液中有CEEF。

Results In the follicles of estrous cycle rat,the signals of MMP 2mRNA were detected in the granulosa cells and thecal cells;w...

结果 在动情周期中，各情期卵巢中卵泡的颗粒细胞和发育良好的卵泡的膜细胞中均可检测到MMP 2mRNA的杂交信号；MMP 9mRNA的表达始于已发育良好的窦前卵泡的膜细胞，此后在小窦状卵泡、窦状卵泡及排卵前卵泡的膜细胞中均检测到MMP 9mRNA的杂交信号。

objective we start with clinical research to explore the ability and mechanism of ion conduction with chinese herbs to curing ovulatory obstacle.methods adopting the methods of clinical observation random contrast.select the cases of ovulatory obstacle dividing randomly into two groups:therapeutic group and control group,the patients of therapeutic group using ion conduction with chinese herbs with act'on nourishing blood and promoting blood circulation to dissipate blood stasis when follicle grow up to 1.4cm.we take the decoction of jia jian tao hong si wu tang conducting the unilateral lower abdomen which has the dominant follicle,treating continuously for 4～8 days and observing ovulation.the patients of control group inject hcg 10000unit when follicle grow up to 1.8cm and observing ovulation.appraising two groups ovulated rate and pregnancy rate,and detect fsh、lh、e2、p at sixteen to eighteen days of menstrual cycle.results ion conduction with chinese herbs can significantly inspires ovulation and its effection is evidently higher than control group and has a higher pregnancy rate,no side effect of ovarian hyperstimulation syndrome.conclusion ion conduction with chinese herbs has significant function on inspiring ovulation.the target effection of treating ovulatory obstacle is exact.this method has a ovulated rate and higher pregnancy rate.

采用临床观察、随机对照的方法，选择排卵障碍的患者，随机分为治疗组和对照组，治疗组在b超监测卵泡生长达到1.6cm时，给予具有养血、活血化瘀作用的加减桃红四物汤水煎剂，用离子导入仪将中药水煎剂导入有优势卵泡的一侧少腹部，连续治疗4～8时天，同时连续监测卵泡排出情况。对照组在b超监测卵泡达到1.8cm时，开始采用hcg10000u肌注，连续b超监测卵泡排出情况。评价两组排卵率及受孕率，同时在月经周期的第16～22天抽血查fsh、lh、e2、p。结果运用离子导入的方法将具有促排卵作用的加减桃红四物汤水煎剂导入有优势卵泡的一侧附件，其促排卵的作用优于用hcg肌注促排卵，而且受孕率高，无卵巢过激综合征之虞。结论中药离子导入具有显著的促排卵作用，其治疗排卵障碍的靶向作用确切，排卵率及受孕率高。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。