卵泡内卵

The decoction did not have the influence regarding to the wet weight of uterus.(2) Wet weight of ovary:Compared with the blank group,the ovary weight of other groups were significantly lighter(p＜0.05).There is two reasons:First, because of decoction intervention ahead of time,it may had the prevention and the treatment dual function;Second,it possibly promoted the ovulation, and caused the liquid discharged from the pouch of the ovary,thus promoted ovary volume and weight decline.(3) Endocrine changes:①The content of androsterone in high dose groups were significantly lower than the low dose groups(p＜0.05),Chinese herbal had certain dose-effect relationship.

上方对于子宫湿重无影响(P＞0.05)。2、卵巢湿重：用药的各组与空白组比较，对于卵巢的重量均有影响(p＜0.05)，考虑有两个原因：第一，由于造模的同时用药，有预防与治疗的双重作用；第二，可能是促进排卵，使囊内卵泡液排出，从而促进卵巢体积及重量的下降。3、内分泌改变：①高剂量组别与低剂量组别比较，在降调总睾酮的作用更大，差异有统计学意义(p＜0.05)，说明中药治疗作用具有一定的量效关系。

The improved puberty PCOS-IR rat model has following characteristic:Retained the high androgen syndrome and/or the high LH syndrome as the endocrine characteristics;Simultaneously it had sugar fat metabolism unusual, such as the insulin resistance,the redemptive high insulin blood sickness as well as cholesterol ascension.Has the abdomen obese characteristic----In particular fat pad which around reproductive organ obvious increased;The ovary increased obviously,Ovarian cortex accumulated,the number in ovary pouch expansion graafian follicle were remarkable increased, ovulation suppression.

一、改良青春期PCOS-IR大鼠模型具有以下特征：1、保留了高雄激素血症和/或高LH血症等内分泌特征；同时有胰岛素抵抗，代偿性高胰岛素血症以及总胆固醇升高的糖脂代谢异常。2、具有腹部肥胖的特征——尤其是生殖器周围脂肪垫明显增加；3、卵巢明显增大，皮质增厚，卵巢内囊性扩张卵泡数目显著增加，排卵抑制。

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF。

Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3-6mm卵泡的卵泡液中有CEEF。

Regulation of follicular development and atresia is a complicated process,including the interaction of endocrine factors,ovarial regulatory factor and nutritional factors during the controlling of the ovocyte fate.

卵泡发育和闭锁的调节是一个复杂的过程，包括内分泌因素、卵巢内调节因子及营养因素在控制卵母细胞的命运中的相互作用。

Do not cause decomposition of one ovulation, mature follicles to a certain stage, clearly highlighted in the ovarian surface, with the proliferation of follicular fluid, internal pressure increases, so that more and more prominent part of the ovarian tissue is thin, the final rupture secondary oocyte cells and their peripheral zona pellucida and corona radiata, together with the discharge of ovarian follicular fluid in the process called ovulation.

不排卵原因分解1、成熟卵泡发育到一定阶段，明显地突出于卵巢表面，随着卵泡液的激增，内压的升高，使突出部分的卵巢组织愈来愈薄，最后破裂次级卵母细胞及其外周的透明带和放射冠随卵泡液一起排出卵巢这一过程称排卵。

The results showed that the Ang II positive staining was observed not only in thecal cells , oocytes and zona pellucida of the follicles at different developmental stages but also in corpora lutea.

结果表明：Ang II分布在不同发育阶段卵泡的内、外膜细胞，卵母细胞及其透明带上，颗粒细胞和卵丘细胞未见着色。

The size of follicle begin primordial follicle to tertiary follicle(1.5～3mm).The number of mitochondrion increased,and the cristae increased.Gogi body change to type,cortical granule increased,and moved behind cytolemma.The proportion of small,middle and large follicles on ovary were 92.51%(92.51±5.87),4.60%(4.60±4.91) and 2.88%(2.88±2.22) respectively.

卵泡在发育过程中，卵泡细胞形成的突起与卵母细胞始终保持密切的联系：卵母细胞从原始卵泡到直径1.5～3mm的三级卵泡，细胞质中线粒体数量由少到多，线粒体内的嵴不断增加，高尔基体由不典型到变成典型，皮质颗粒由开始形成到形成较多的皮质颗粒，并逐渐地迁移到卵母细胞膜下。

Results The expression of Foxo3a, Bim, FasL in oocyte nests and primordial follicles were detected in the nuclei and cytoplasms of some oocytes. The patterns of Bim and FasL positive rates were consistent with that of Foxo3a and TUNEL positive oocytes rates, which reached the peak in the ovaries of 2-day-old rats.

结果 免疫组化显示Bim和FasL都在新生大鼠部分卵母细胞巢和原始卵泡的卵母细胞核内和胞浆高表达，且阳性率曲线都在2d达到高峰，随后逐渐降低，与TUNEL和FoXo3a的阳性率变化曲线一致。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。