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It changed to be strong reflection after degeneration.2.On the growth of follicle,follicular cell produced prominency which keeps touch with oocyte rightly.

在发情周期内,有大小不一的发育卵泡和黄体;卵泡显示为无回声的团状黑影;黄体为均匀的深灰色细小颗粒组成,退化后成为强反射。2。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后,分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h,研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

While increased developing follicles, mature follicles, normal strome were observed in treatment group.

干预组各级发育卵泡增多,间质正常,可见成熟卵泡

After puberty until menopause, several follicles in the growing pool are recruited in each ovarian cycle with granulosa and thecal cells proliferation and differentiation.

到青春期前,绝大多数卵泡(50%-70%)在各个阶段发生退化而闭锁,仅有少数能发育成排卵前卵泡

The results showed that Yiweitang, which could tonify YangMing Fluid and Qi, could change the growth internal environment of follicle, promote the growth of follicle, increase the excretion of ovary inhibin and decrease FSH, improve the blood supply of ovary, increase the expression of hormone receptor and restrain ovary apoptosis.

实验研究提示益胃汤具有改善初老雌性大鼠卵泡生长的激素内环境,促进卵泡生长,增加卵巢抑制素的分泌,抑制FSH,改善卵巢血供;提高激素受体表达及抑制卵巢细胞凋亡等作用。

(1)FM with an incidence of 27.0% might be an important cause of infertility.(2) FM results finally in follicular atresia or multiple immature luteinized follicles.

(1)FM是不孕的一个重要原因;(2)FM结局有二,一为卵泡闭锁,另一为未成熟卵泡黄素化。

Materials and Methods: We got the follicle fluid when eggs were withdrawed during IVF cycle and then separated and cultured luteinized granulosa cells using serum free medium.

IVF周期穿刺卵泡取卵时所得卵泡液,进行颗粒细胞分离培养,在无血清培养体系中加入不同浓度VEGF,观察基础状态下或HCG作用下培养液中〓、P浓度的变化。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of IVF-ET program and were cultured with M199 media plus various concentration of follicle stimulating hormone (FSH, 0% 0.5% 5% 10% 25% 50) or human chorionic gonadotropin(hCG, 0% 5% 10% 50% 75% 100IU/ml). The media were collected and PAPP-A in the media were measured by time-resolved fluoroimmunoassay.

将来自体外受精—胚胎移植的卵泡黄素化颗粒细胞分离纯化后,利用不同剂量促卵泡激素(FSH,0、0.5、5、10、25、50)或人绒毛膜促性腺激素(hCG,0、5、10、50、75、100IU/ml)刺激体外培养的颗粒细胞,收集培养液,采用时间荧光免疫分析法测定培养液中PAPP-A的量。

Follicle is FSH and luteinizing hormone under the development and maturation.

卵泡是在促卵泡成熟激素和黄体生成素(LH的作用下发育成熟的。

The number of the medium sized follicles (diameter=3~5 mm) was highest (8 5/ovary) at diestrus and lowest (0/ovary) in metestrus.

中等卵泡数量在发情间期最多,为8.5个/卵巢,在发情后期卵巢中无中等卵泡

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