卵泡

There were many granulosa cells undergoing apoptosis in the atretic follicles in the pig.

猪闭锁卵泡中存在严重的颗粒细胞凋亡现象。

The percentage of apoptotic granulose cells in the healthy, slightly atretic and atretic follicles were 0.13±0.02, 0.32±0.02 and 0.51±0.02, respectively.

健康、轻度闭锁以及闭锁卵泡中颗粒细胞凋亡的比例分别是0.134±0.02，0.32±0.02和0.51±0.02。

Methods: A dual-chamber culture system was prepared. Porcine ovarian follicles were divided into healthy and atretic groups. Granulosa and thecal cells were isolated, and cultured with gonadotropin in the dual-chamber system. After 48 hours, angiotensin II, renin activity, estrodiol(E2), progesterone and testosterone in the media were determined by radioimmunoassay.

制备人胎盘羊膜双池培养系统，分离了猪卵巢颗粒细胞和卵泡膜细胞，并分为健康和闭锁两组，在促性腺激素刺激下协同培养。48 h后应用放射免疫测定法测定两种细胞培养液中的血管紧张素II、肾素活性、雌二醇(E2)、孕酮以及睾酮水平。

The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

应用该技术对猪、山羊和牛不同大小、不同健康程度卵泡及体内和体外成熟过程中卵母细胞染色质构型的变化发现：1）不同动物卵母细胞染色质构型不同；猪和牛卵母细胞染色质围绕核仁凝集形成环，但山羊卵则没有此环。2）卵母细胞在GVBD之前都同步在某一染色质构型；猪同步在GV1，山羊GV3n，牛GVf，说明这些染色质构型代表着进行性变化。3）染色质构型的变化反映基因转录活动的变化。4）染色质构型受环境变化的影响。

It can lower the levels of follicle stimulating hormone and luteinizing hormone in blood serum and inhibit ovulation, thus causing infertility. Due to its effect of lowering chorionic gonadotropin, restraining the development of corpus luteum graviditatis and iterfering the growth of uterus and the supply of embryotrophy Lithospermum has been confirmed to be effective in termination of pregnancy and herb abortion.

紫草能降低体内卵泡刺激素和黄体生成素水平，抑制排卵从而导致不孕；可降低血清人绒毛膜促性腺激素含量，破坏绒毛生长，阻碍妊娠黄体的发育，起到终止妊娠及辅助药物流产的作用。

For exploring the endocrinal mechanism of sika deer\'s reproduction, four hormones(follicle stimulating hormone FSH, luteinizing hormone LH, estradiol e2 and progesterone P ) after weaning(from August 22nd to October 3rd ) and during estrus period (from the 16th day pre estrus to the 6th day after estrus)were tested.

为了进一步的探索梅花鹿的生殖内分泌机制，本论文对雌性梅花鹿在断乳后和发情前后两个时期血清中四种主要生殖激素(促卵泡素FSH、促黄体素LH、雌二醇E_2和孕酮P)进行了测定分析。

Based on pre-experiment foundation of the earlier period, 60 male patients among 45-65 year old were chosed as the clinical case according to the PADAM diagnosis standard, and were divided into 3 groups according to the block stochastic principle, the observation group (acupuncture combined with BUSEHN capsule), group 1, group 2 (takes orally BUSEHN capsule group), each group had 20 cases, and each group of patients was measured by the method of radio-immunity for the serum level of testosterone, luteinizing hormoha, follicle-stimulating hormone before and after the treatment. By the contrast of the grade of PADAM symptom among three groups before and after treatment ,we can reveal the whole funcamental characteristic of acupuncture combined with herb ,and discuss a new way to enhance the curative effect of acupuncture ,and summarize the endocrinous adjustment of acupuncture for the PADAM's patients and the orchidic improvement by the treatment of acupuncture.

在前期预实验的基础上，选择符合诊断标准的PADAM诊断标准的45-65岁男性患者临床病例60例，按照区组随机原则分为3组，观察组、对照组1、对照组2，每组各20例，采用放射免疫法分别测定各组患者治疗前后血清中血清睾酮、黄体生成素、卵泡刺激素的血清浓度的水平；通过三组及三组间治疗前后PADAM症状评分指数的对比研究，揭示针药结合的整体调节作用特色，探讨提高针灸疗效的新途径；总结出针刺对中老年雄激素缺乏综合征患者的内分泌的调节及针刺对睾丸的改善程度，探讨针药结合治疗本病的机制及针灸增效剂的应用前景，为今后进一步研究打下坚实基础。

Ets-1 may play important roles in the regulation of follicular development and luteal function during the estrous cycle.

Ets-1可能在大鼠动情周期中的卵泡发育、黄体生成及退化过程中起着重要的调节作用。

Histological structure of follicular system in yaks during the estrous cycle was similar to that of cattle and buffalo.

结果表明，牦牛发情周期中卵巢卵泡发育的组织结构与其他牛基本相似。

The changes of follicle development and steroid production during estrous cycle and their relationship.

发情不同时期卵泡发育、类固醇激素分泌以及颗粒细胞凋亡的变化规律以及相互关系。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。