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The decoction did not have the influence regarding to the wet weight of uterus.(2) Wet weight of ovary:Compared with the blank group,the ovary weight of other groups were significantly lighter(p<0.05).There is two reasons:First, because of decoction intervention ahead of time,it may had the prevention and the treatment dual function;Second,it possibly promoted the ovulation, and caused the liquid discharged from the pouch of the ovary,thus promoted ovary volume and weight decline.(3) Endocrine changes:①The content of androsterone in high dose groups were significantly lower than the low dose groups(p<0.05),Chinese herbal had certain dose-effect relationship.

上方对于子宫湿重无影响(P>0.05)。2、卵巢湿重:用药的各组与空白组比较,对于卵巢的重量均有影响(p<0.05),考虑有两个原因:第一,由于造模的同时用药,有预防与治疗的双重作用;第二,可能是促进排卵,使囊内卵泡液排出,从而促进卵巢体积及重量的下降。3、内分泌改变:①高剂量组别与低剂量组别比较,在降调总睾酮的作用更大,差异有统计学意义(p<0.05),说明中药治疗作用具有一定的量效关系。

Objective: To investigate the relationship between the renin-angiotensin system and steroidogenesis during the follicular development and atresia.

目的:研究在卵泡发育和闭锁过程中,卵巢的肾素-血管紧张素系统及其与甾体激素生成的相互作用。

METHODS Human secondary follicles were isolated from ovarian tissues obtained from cancer patients and grown in vitro within a bio-engineered culture system for 30 days.

方法从癌症患者的人卵巢组织中分离出次级卵泡,并在体外的生物工程培养体系中培养30天。

Apoptosis of in vitro cultured granuiosa cells was significantly suppressed by IGF-Iand the effect was dependent on concentration.

IGF-I可以显著抑制体外培养的卵泡及颗粒细胞的细胞凋亡,并且同样具有剂量效应,IGF-I浓度为50ng/ml时效果最好。

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术,观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响,结果证实了促孕液治疗卵巢静止、持久黄体的机理:(1)微弱的雌激素样作用,能刺激卵泡发育,促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化;(2)提高子宫内膜碱性磷酸酶的活性;(3)改善微循环;(4)促进子宫运动。

Until now, there is no article about the expression and regulation of the actin depolymerization factor/cofilin during follicular development in mammal.

目前关于AC蛋白家族在哺乳动物卵泡中的表达还没有报道。

Techniques in embryo technology (such as in vitro production of embryos and animal cloning) need large quantities of high quality oocytes. But the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. It is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo.

目前胚胎工程技术研究和开发(如体外生产胚胎和体细胞克隆等)需要大量高质量的成熟卵母细胞,但利用屠宰动物卵巢卵母细胞经过体外成熟培养而获取的卵母细胞质量还远不如体内成熟卵母细胞,其原因一般认为是由于缺乏体内主卵泡阶段的获能作用。

Methods: The serum estrin (E2), progestogen, follicule stimulating hormone and luteotropic hormone were measured in 15 patients with hysterectomy, 22 patients with uterine arterial embolization and 29 normal persons, respectively.

对15例子宫切除患者、22例子宫动脉栓塞患者和29例正常人分别测定血清雌二醇、孕酮、卵泡雌激素和黄体生成素。

Methods The microorganism of macula retinae on s kin lesion in 26 male patients and the serum follicular-stimulating hormone,lateinizing hormone,progestrong and estriol prolactin in 25 men were st udied with quantitive, positioning, qualitative and radio-immunitive methods, a nd then compared the results with those of the healthy men.

分别用定量、定位、定性和放射免疫学方法对26例男性黄褐斑患者皮损区微生态和25例血清卵泡刺激素、孕酮、雌三醇(E3)、黄体生成激素及催乳激素的水平进行研究,并将其结果与健康男性面部菌群、血清性激素水平加以比较。

After being dispersed by simply beat upon with syringe and adherent culture, the mature cumulus cells from mature OCCs in COH cycles, together with growth factors in the follicular fluid or extraneously supplemented, could promote the IVM of immature oocyte.

来自控制性促排卵周期的成熟卵丘细胞经简易吹打分散后贴壁培养,可能能协同卵泡液中或外加的生长因子,促进未成熟卵母细胞的体外成熟,而本研究技术简易有效,可用于挽救促排卵周期的未成熟卵。

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