卵母细胞

It is concluded that (1) there are no relationships between fusion rate and relativeness of the recipient cytoplasm to nucleus donor cells,(2) cytoplast of the goat MII oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species,(3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos.

由此结果得出以下结论：(1)山羊M II期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率；(2)山羊M II期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育；(3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

A cell from which gametes develop by meiotic division, especially a spermatocyte or an oocyte.

配子母细胞，配子细胞配偶子通过配子减数分裂而发展的细胞，尢指精母细胞或卵母细胞

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

A pure and single K+ current of ultrarapid delayed rectifier K+ current or transient outware K+ current is respectively expressed on Xenopus Oocytes. This is a moderm pharmacological model for evaluation class Ⅲ antiarrhythmiv durgs, overcomes many defcts such as lacking human fresh cardiac muscle cells as material and co-expression of many currents on cell membrane. A new compound A1998 from Ocean material is evaluated in this model for class Ⅲ antiarrhythmic functions.

5和Kv4.2cDNA转录为mRNA，然后将其分别注入蟾蜍卵母细胞上：在蟾蜍卵母细胞上分别获得纯净、单一的超速延迟性整流钾电流(Ikur，ultrarapid delayed rectifier K+current)和瞬间外向钾电流(Ito，transient outward K+current)表达，克服以往在筛选评价抗心律失常药物时，人新鲜心肌细胞取材困难、多种电流在细胞膜表面共同表达等缺点，从而建立评价Ⅲ类抗心律失常药物的先进药理模型，并筛选具有Ⅲ类抗心律失常药理作用的新化合物A1998。

This thesis compared the effect of Uracil, ITS, and ATP on the maturation of bovine oocytes and further development, observed the microstructure changes of oocytes and parthenogenetic embryos among different groups, and granule cell apoptosis among different groups.

本文对比了添加尿嘧啶和三价因子等试剂对牛卵母细胞成熟及后期发育的影响、观察了不同组之间成熟牛卵母细胞和孤雌激活胚胎显微结构变化和不同组之间颗粒细胞凋亡情况。

The meiosis of oocytes differ from mitoses of general cells. Especially in most mammalian species, oocytes are formed during fetal life, but they are arrested at the prophase stage of the first meiotic division until around the time of ovulation.

卵母细胞的成熟分裂有别于一般细胞的有丝分裂，尤其是哺乳动物，在胎儿期或出生后不久，初级卵母细胞就陆续进入第一次成熟分裂前期阶段，但长时间的&休止&于此期，直到性成熟时在促性腺激素等因素的作用下，第一次成熟分裂才重新启动。

Resembled to control oocytes, MAPK was inactivated after cytoplasts activation. However, after activation the cytoplasts showed fragmentation while pronuclei were formed in the control oocytes. This indicated that nuclei play important role in maintainence of oocytes normal morphology.

成熟胞质的活化与卵母细胞相似，在活化后MAPK被迅速灭活，只是胞质在活化处理后均发生碎裂，而卵母细胞活化后形成原核，这表明核在细胞形态维持中起重要作用。

The results showed that chromium trichloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF and the number of oocytes of superovulation, inhibit the germinal vesicle breakdown of mouse oocytes in vitro culture, but little impact on GVBD of mouse oocytes in vivo .

结果表明，三氯化铬可以抑制卵母细胞第一极体的释放，降低小鼠超排卵数和卵母细胞的存活率和体外受精率。

With immunofluorescencecytochemistry and confocal microscopy,we found there were a lot of MAPK from 0-hour GV oocytes to 44-hour MII oocytes,and no active MAPK in 0-hour GVoocytes and no MAPK in the germinal vesicle.

通过体外培养猪卵巢卵母细胞至完全成熟，在培养一定时间取样用免疫荧光化学与激光共聚焦显微术结合的方法观察MAPK和p90rsk在卵内的分布规律，研究了猪卵母细胞中MAPK和p90rsk的表达和定位。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。