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So it is important to study the mechanism of oocyte aging. Herein, we studied the change of oocyte chromosome after ovulation into the oviduct in vivo.

这种卵母细胞的老化会导致包括人在内的哺乳动物的胚胎发育异常,所以很有必要研究排卵后卵母细胞的老化机理。

Before ovulation, primary oocytes complete the first meiosis produce a secondary oocyte and a polar body.

在排卵前,初级卵母细胞完成第1次减数分裂产生次级卵母细胞和第1极体。

A lot of follicular compartment cells, include cumulus cells, theca cells, granulosa cells cooperate with each other to facilitate the maturation of oocytes.

卵母细胞的成熟过程至少需要经过周围卵丘细胞、卵母细胞核以及核外细胞质等几个方面一系列的变化过程。

The rate of Flow cytometry to analyse retinoic acid haploid is 19.3%.The sperm-like cells achieved could activate bovine oocytes by sperm injection, and the development rate of blastosphere was 20.36%. Conclusions SSCs of newly -born mice can be induced and differentiated into sperm-like cells and are also capable of activating oocytes.

本实验通过体外诱导新生小鼠SSCs分化为精子样细胞,探讨不同诱导方法的诱导效率;采用显微注射法将精子样细胞注射到卵母细胞和去核卵母细胞,探讨其体外重构胚的发育能力,为SSCs体外分化研究打下基础。

Methods A C57BL/6j mouse cumulus cell nucleus 10-12 mm in diameter was inserted into the perivitelline space of an enucleated oocyte.

方法将直径10~12mm的C57BL/6j小鼠卵丘细胞核注射到去核卵母细胞透明带下,构建供体核-卵母细胞复合体。

The feasibility of maturing denuded buffalo oocytes in vitro was explored in this study, so as to provide a model to investigate the mechanisms of oocytes maturation.

主要探讨无卵丘水牛卵母细胞体外成熟的可行性,以便为研究卵母细胞成熟机理提供模型。

Rates of fertilization and morulae/blastocysts in oocytes with fully expanded cumulus were significantly higher than those in oocytes with poorly expanded cumulus.

扩展良好的山羊卵巢卵母细胞的受精率和胚胎发育能力显著高于卵丘扩展差的卵母细胞

The results show the Boer goat oocyte and pre-implantation embryo are telomerase positive. According to the densitometry of bands under the CCD imagine system, the total telomere repeats product generated of oocytes, 4-cell, 8-cell, morulae and blastocyst are 25.348, 273.832, 56.117, 251.118, 519.46, respectively.

结果表明波尔山羊卵母细胞和早期胚胎都是端粒酶阳性;依据电泳条带在凝胶成像系统下的光密度计算相对总产品产量定量比较发现,卵母细胞最低为25.348,4-细胞为273.832,至8-细胞又降低到56.117,随后快速升高,桑椹胚为251.111,囊胚端粒酶活性最高为519.46。

In conclusion, season affected both quantity and quality of superovulated rabbit's oocytes.

所以,季节既影响超数排卵兔的卵母细胞数,也影响卵母细胞的质量。

This study compared outcomes of in vitro maturation and in vitro fertilization intracytoplasmic sperm injection cycles after IVM of immature germinal vesicle oocytes.

研究对比体外受精-单精子卵胞浆内注射周期所获生殖泡期不成熟卵母细胞以及体外成熟周期所获GV期卵母细胞体外培养成熟后的胚胎发育情况。

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