卵母细胞

METHODS: Virginal and multiparous rabbits were superovulated by folliclestimulating hormone at different concentrations. After being obtained, the mature oocytes were activated by electric current with different strength.

采用不同剂量的垂体促卵泡素对青年兔和经产兔进行超数排卵，获得兔卵母细胞后，以不同强度的直流电脉冲对兔卵母细胞进行孤雌激活。

Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取从屠宰场获取的猪卵巢中的卵母细胞，在加入和不加hCG和PMSG的mTCMl99培养液中分别培养22小时，地衣红染色发现77.6％的卵母细胞达到MII期；去卵丘细胞后在mTBM中用鲜精或冻精解冻后受精6小时，在NCSU23中培养14小时后固定染色发现受精率分别为87.5％和64.1％；电激活后在NCSU23中培养20小时后固定染色发现原核形成率为94.1％。

The results indicated: 1 .There is obvious difference between the gonads of precocious and unprecocious fish: in female, the ovaries of unprecocious fish stay in 141 stage, and are mainly consisted of oogonias and early oocytes; the ovaries of precocious fish have developed into IIIAV stage, most of the oocytes are in the large growth stage.

性早熟与未早熟大黄鱼性腺的发育有显著不同：未早熟鱼卵巢停留在Ⅰ～Ⅱ期，卵巢内主要由卵原细胞和早期卵母细胞所组成；性早熟大黄鱼卵巢已发育至Ⅲ～Ⅳ，卵巢内大多数卵母细胞处于大生长期中期或后期。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示，在卵母细胞和8细胞期胚胎，基因calm3和trm112l的表达量无显著性差异，而在囊胚期的表达量与前两个时期的表达量存在显著性差异，推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控，提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

The results showed that immunopositive substance existed in the ovary and testis of different stages. At the early developmental stage of ovary, the cytoplasm and nucleolar membrane of oogonia and early primary oocyte showed immunopositive reaction with strong or medium intensity to 17 b -estradiol, testosterone and progesterone antibodies.

在卵巢发育早期，卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应；在大生长期和成熟期，卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱，而对雌二醇则显著增强。

Newly ovulated mouse oocytes were cultured in the CZB medium supplemented with different concentrations of pyruvate before treated for activation or assayed for MPF activity.

我们猜测卵母细胞在不含丙酮酸的条件下老化的更快，并且卵丘细胞可能通过消耗不含葡萄糖的CZB中的丙酮酸从而促进卵母细胞老化。

When oocytes were matured at 37-39℃,the appearance of cumulus cell had protective effect on cleavage and blastocyst rate,the development rate of DOs significantly decreased compared to COCs.

当卵母细胞在37-39℃成熟培养时，卵丘细胞对卵母细胞的发育有一定的保护作用，DOs的发育能力显著低于COCs。

Also, the intensity of immuno-positive reactions to them enhance with the development of the gonads.

免疫组织化学法也显示卵原细胞、卵母细胞、精原细胞、精母细胞和精子细胞存在睾酮和17β-雌二醇的免疫阳性物质，而且睾酮和17β-雌二醇的免疫反应强度也是随着性腺发育成熟而增强。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体，昆明白小鼠MⅡ期卵母细胞为受体，研究了3%蔗糖处理卵母细胞去核后，供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。