卵母细胞

It showed that the cumulus cells did play significant role in the stimulation of nuclear maturation of porcine oocytes and subsequent development.

表明卵丘细胞对猪卵母细胞的核成熟及卵母细胞体外受精后的卵裂和早期胚胎发育，都具有明显的促进作用。

In ovary, PNA receptors appeared in the oocyte cytoplasm of second phases of oogenesis; the positive granules gradually increased from third to forth phases, and they exhibit a maximum expression before vitellogennic stage in cytoplasm of oocyte; from vitellogennic stage to chorionation stage, the positive granules gradually reduced. Binding sites on follicle cells were changed with their morphological variation in every stage of oogenesis.The staged and specific expression of oncogene c-kit, the tyrosine-kinase receptor, is closely related with gametogenesis.

2在中华蚱蜢卵子发生的早期，PNA受体是由卵母细胞自身合成；在卵黄发生前的准备时期，卵母细胞质中的PNA受体一部分来源于自身合成，另一部分则来源于滤泡细胞，而糖复合物的大量出现可能直接与卵黄发生有关；卵黄发生期PNA受体的减少可能是在卵黄物质形成中部分发生了修饰；卵黄和卵壳的相继形成过程中，阳性反应的出现说明PNA受体参与了卵黄膜及卵壳的形成，而这些受体物质是由滤泡细胞分泌的。

The results show that, with the maturity of the gonad, the undifferentiated germ cell divided and differentiated to smaller oogonium or spermatogonia, and the oogonium or spermatogonia transformed to oocyte and sperm undergoing a series of maturation process.

摘 要 通过观察栉江珧的未分化生殖细胞，精原细胞和卵原细胞，精母细胞和卵母细胞，精子和卵子的形态结构与分布状况及核仁在卵成熟过程中的变化，对栉江珧生殖细胞发生及成熟过程的有关问题进行了探讨。

The results indicate that:(1) For consuetudinary maturation culture mediums, such as M199 andα-MEM,the maturation rates of denuded oocytes are significantly lower than Cumulus-oocytecomplexs.

结果表明：(1)对于常规的成熟培养液M199、α-MEM而言，去除卵丘细胞的卵母细胞成熟率要极显著低于卵丘卵母细胞复合体。

Xpression of TGFαand EGFR protein in oocytes, preimplantation embryos and reproductive tracts by immunohistochemy 2.1 Expression of TGFαand EGFR protein in oocytes and preimplantation embryos The results showed that expression of EGFR and TGFαwere in immatured and matured oocytes, cumulus cells, fertilized oocytes and different stage of preimplantation embryos.

GFR和TGFα在未成熟卵母细胞、卵丘细胞和成熟卵母细胞的细胞膜区域均有表达，TGFα在受精卵中的表达量明显增加。

Staining analysis for porcine oocytes was conducted by using 0.1% Orcein solution in order to investigate the role of cumulus cells in nuclear maturation of porcine oocytes.

用0.1%地衣红对猪卵母细胞进行染色检查，分析不同层数卵丘细胞对卵母细胞成熟的影响。

In this thesis, we find that dlg is indispensible in the establishment of anterior-posterior and dorsal-ventral polarity of drosophila oocyte. Removal of Dlg function from the posterior follicle cells using the FLP/FRT system leads to disruption of oocyte skeleton reconstruction that is elicited by the failure of those posterior cells to differentiate normally in mid-oogenesis. We demonstrate that abnormity of Notch, JAK-STAT and EGFR signal pathway in dlg mutants contributes to this aberrant differentiation. dlg null mutant also blocks the normal differentiation of two groups of anterior follicle cell-stretched cell and centripetal cell, but not border cell, with a lower penetrance. However unlike the result in posterior follicle cells, Notch and JAK-STAT signaling are both undisrupted in all mutant anterior follicle cells, implying other fate determinants may be involved.

我们的研究发现，后端滤泡细胞中的Dlg在果蝇卵子发生中期卵母细胞前后轴和背腹轴建立过程中也是必须的，PFC中dlg完全缺失型突变引起PFC的分化异常，导致卵子发生中期卵母细胞骨架重组异常，Stau、Grk等极性决定蛋白定位错误。dlg突变阻碍了Notch、JAK-STAT、EGFR等调节PFC分化的信号通路的激活。dlg突变的PFC也没有获得前端滤泡细胞命运。dlg突变不影响前端滤泡细胞群中边界细胞的分化，但是在一定程度上影响伸展细胞和向心细胞的分化，并且这种影响不依赖于前端滤泡细胞Notch或JAK-STAT信号激活的异常。

Understanding the role of cumulus oophorus in oocyte maturation will enable us to reveal the mechanism of mammal ooc.

卵丘是指在卵母细胞外周并与之进行代谢联系的颗粒细胞群；卵丘对于卵母细胞成熟有极其重要的作用。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。