卵母细胞

The results indicate that:(1) Using SUZI method can improve the fertilization rates of denuded oocytessignificantly. This result suggests zona pellucida may be one of the reasons that makedenude oocytes matured in vitro have a clearly deficiency on fertilization in vitro.

结果表明：(1)利用精子带下注射技术可以极显著的提高去除卵丘细胞的卵母细胞的受精率，表明透明带结构的改变是去除卵丘细胞的卵母细胞存在受精障碍的重要原因之一。

A correction factor for vitellogenic atresia was included based on the level of atresia and the size of atretic oocytes in relation to normal oocytes finding that OPD would be biased when smaller atretic oocytes are present but not accounted for.

卵黄闭锁的相关因素包括闭锁水平和与正常卵母细胞相关的闭锁卵母细胞的大小，发现当较小的闭锁卵母细胞出现而不计算在内时 OPD 会有偏差。

The duration and temperature during ovary transportation and handling might, therefore, affect the recovery of culturable COCs, chromatin configuration and developmental competence of oocytes.

在具体的实施过程中，从屠宰场向实验室运输卵巢、卵母细胞培养前操作等都需要一定的时间，这可能会对卵母细胞的回收率、卵母细胞成熟质量、甚至受精后的发育产生不良影响。

After that , the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article , in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos .

和胞浆内单精子注射两种方法对玻璃化冷冻卵母细胞的受精能力进行了比较研究，旨在建立能够进一步提高牛冷冻卵母细胞受精能力的改进方案，使卵母细胞冷冻技术上升到与胚胎冷冻同样成熟的水平。

After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻；采用最小样本量玻璃化冷冻方法进行冷冻；在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

为探讨Dynein与卵母细胞减数分裂错误形成的关系，采用小鼠卵母细胞体外培养成熟技术和半定量RT-PCR方法，分别检测Dynein抑制剂-原钒酸钠对卵母细胞成熟率和cyclin B1基因转录水平的影响。

In the PbⅠ stage oocyte, perivitelline enlarged further, the cumulus cells expanded and the cell junctions disappeared. On the membrane of oocyte near the first polar body, a no microvillus area was observed, where probobly was the position for the second polar body extruding.

在PbⅠ阶段的卵母细胞内，卵周隙进一步扩大，卵丘细胞扩散，无细胞间连接存在，卵母细胞表面靠近第一极体的部位存在无微绒毛的区域，可能是第二极体释放的部位。

The results of the determinating the activity of the CA in vitro showed that the CA of the water—fed individuals were in activing statics after eclosion. Because of the apperence of the Vg in haemolymph and the deposition of the vitellin in oocytes in the unfed females, it was shown that the CA in such individuals were in activiting statics, too. Again because in the water—fed females which did not lack the feeding action the activity of the CA showed dropping tendency gradually still, it could be said that the activiting of the CA was possibly effected by the nutrition but it was never related to the adult feeding action. In another respect, the brain of the female adult had not significant effect on the activiting CA. The oocytes could depositing vitellin in individuals removed brain tissue on the day of eclosion, which showed that the activting of CA was not concerned with brain.

CA活性的体外测定说明饲水个体的CA同样具有活性，不取食个体血淋巴中Vg的存在以及卵母细胞中卵黄的沉积表明，在不取食的个体中CA同样具有活性，所以CA的活化与成虫期的取食动作无关，与成虫是否取食花蜜也无太多的联系，但是CA活性的维持可能受成虫期营养的影响，如果以卵母细胞发育和CA体积大小作为CA活性的指标，则饲水个体CA的活性在后期受到了影响，说明饲水不能替代取食蜂蜜来维持成虫期CA的活性；成虫期脑对CA的活化无明显作用，羽化当日摘除脑的个体，其卵母细胞仍然可以沉积卵黄，说明CA的活化与脑无关，而是受某种不确定的因子的影响；用抗JH物质—早熟素—Ⅱ以10ug／虫的剂量在颈部点滴处理雌虫，对Vg的发生和卵巢的发育具有一定的迟缓作用，其原因有待于进一步的研究。

The size of follicle begin primordial follicle to tertiary follicle(1.5～3mm).The number of mitochondrion increased,and the cristae increased.Gogi body change to type,cortical granule increased,and moved behind cytolemma.The proportion of small,middle and large follicles on ovary were 92.51%(92.51±5.87),4.60%(4.60±4.91) and 2.88%(2.88±2.22) respectively.

卵泡在发育过程中，卵泡细胞形成的突起与卵母细胞始终保持密切的联系：卵母细胞从原始卵泡到直径1.5～3mm的三级卵泡，细胞质中线粒体数量由少到多，线粒体内的嵴不断增加，高尔基体由不典型到变成典型，皮质颗粒由开始形成到形成较多的皮质颗粒，并逐渐地迁移到卵母细胞膜下。

Membrane potential, intracellular Na+, K+, Cl, H+ activities and Na+, K+ transference number in toad oocytes were measured in vitro by using conventional and ion-sensitive microelectrodes during the insulin-induced oocyte maturation.

近年来，胰岛素及胰岛素样肤类物质在卵母细胞成熟中的作用越来越引起人们的关注。EI一Etr等(1979)用非洲爪蟾卵母细胞作实验，发现胰岛素及其类似物具有促进卵母细胞成熟分裂的作用。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。