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卵母细胞

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Methods: Microinjection technique was used to structure the human SERT heterologious system.

显微注射方法将 hSERT 基因 cRNA 注射到非洲爪蟾卵母细胞内,建立异体表达系统。

In this study, we examined the effect and the mechanism of ANP on porcine oocyte maturation, fertilization and giant panda spermatozoa function.

本文以猪和大熊猫为对象,研究了ANP在卵母细胞成熟和精子功能中的作用及机理。

Antinori made his mark in the late1980s,when he pioneered a technique called subzonal inseminationto position sperm below the zona pellucida,the barrier around the egg,or oocyte.

运用该技术,可以把精子放置在卵子或卵母细胞的屏障清澈层下。

For the Batrachuperus tibetanus, the follicle cells are the main sites for e2, P and T, but the thecal cells are not shown to product steroid.

说明,在卵母细胞发育中,有尾两栖类和无尾两栖类性类固醇激素的作用有一些差异。

The reprogarmming of embryonic genome is likely the molecular foundation of the transition of highly differentiated oocytes into totipotential embryonic blastomeres.

基因表达的&再程序化&可能是高度分化的卵母细胞转变成胚胎中具发育全能性分裂球的分子基础。

In stage Ⅰ and Ⅱ oocytes, strong hybridization signals of vasa RNA were dispersed uniformly throughout the cytoplasm.

在Ⅰ、Ⅱ期卵母细胞中vasa RNA的杂交信号强烈,均匀地分布在整个胞质。

At present, the research mainly concentrates on vitellin purification and characterization, vitellogenin synthesis and hormonal regulation, vitellogenin absorption and processing.

目前,对蜱类卵黄发生的研究主要包括卵黄蛋白的纯化与鉴定,卵黄原蛋白的合成与调控,卵母细胞对卵黄原蛋白的摄取以及转变为卵黄蛋白的分子过程。

Both the proteins components contained in haemolymph, fat body and ovary and the kenetics of the vitellogenin in haemolymph and ovary were analyzed by SDS—polyacrylamid electrophoresis, Ouchterlony plate tests and Rocket—immuno—electrophoresis. The results showed that the vitellogenesis initiated in adult stage and the Vg in haemolymph was detected firstly at 48h after eclosion. The titer of Vg in haemolymph raised rapidly in following 3 days and almost at the same time the vitellin appeared in oocytes. It was taken about 4—7 days after eclosion for the first eggs to grow up to the matured statics and to be laid out.

SDS-PAGE,双向免疫扩散和RIE分析粘虫生殖周期中血淋巴,脂肪体和卵巢中蛋白质谱带以及Vg滴度的变化,结果表明,Vg发生始于成虫期,Vg在血淋巴中的首现时间在羽化的24小时以后,以后连续三天血淋巴中的Vg滴度迅速上升,与此同时,开始沉积卵黄,卵母细胞逐渐发育长大,到成熟排出体外时大约历时4-7天。

METHODS: 12 GABAA and 1 glycine receptors were expressed in Xenopus laevis oocytes and studied using two-electrode voltage clamping.

12 GABAA 和 1甘氨酸受体能在非洲爪蟾卵母细胞中表达并且通过双电极电压钳夹法研究测得。

The results suggested that pH decreasing inhibited the ATP-activated current mediated by rat P2X_4 receptors expressed in Xenopus oocytes.

pH降低对表达在爪蟾卵母细胞上的大鼠颈上神经节神经元P2X_4受体的激活电流具有抑制作用。

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