卵母细胞

Follicular oocytes were recovered by aspiration and dissection from a giant panda died of disease during nonbreeding season and fertilized in vitro with frozen semen after maturation in vitro.

用抽吸法和切割法采集非发情季节病亡大熊猫的卵泡卵母细胞，经体外成熟培养后，用大熊猫冷冻精液进行体外受精。

All results above indicated that:The expression of ERβand p-ERβin mouse ovary at oestrous cycle and in early gestational period changed regularly, it showed that ERβand p-ERβhad important effects to ovaries development, ovarian follicles groth, oocyte maturation and form corepus luteum in female mouse ovary during oestrous cycle and in early gestational period.

以上结果表明：ERβ及其磷酸化形式——p-ERβ在小鼠发情周期以及妊娠早期卵巢中表达有规律性的变化，揭示ERβ与p-ERβ在雌性小鼠发情周期及妊娠早期，对卵巢发育、卵泡生长、卵母细胞成熟和黄体形成有重要的作用。

The result showed that different concentration of FSH and LH were needed for sheep oocytes IVM in different season Xinjiang. FSH 12.9ug/ml,LH0.86IU/ml is proper to sheep oocytes IVM in summer-autumn anestrous period. FSH 8.6ug/ml,LH0.57IU/ml is proper to sheep oocytes IVM in autumn oestrous period.

结果表明：新疆绵羊卵母细胞在不同的季节中，体外成熟所需的FSH、LH浓度不同，夏秋乏情季节适宜于体外成熟的激素浓度为FSH 12.9ug/ml，LH0.86IU/ml；秋季发情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml，LH0.57IU/ml；春季乏情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml，LH0.86IU/ml。

Orcein staining;oocytes;nuclear maturation;swine

地衣红染色；卵母细胞；核成熟；猪

Regulation of follicular development and atresia is a complicated process,including the interaction of endocrine factors,ovarial regulatory factor and nutritional factors during the controlling of the ovocyte fate.

卵泡发育和闭锁的调节是一个复杂的过程，包括内分泌因素、卵巢内调节因子及营养因素在控制卵母细胞的命运中的相互作用。

Findings as follows: Experiments 1: Hormone to ovocyte IVM mature ratio influence: Dyes with H33342 observes COCs in three kind of different culture environment FSH → without hormone,FSH→ LH, FSH + LH → without hormone.""→""

研究结果如下：试验1：用H_(33342)染色观察COCs在将卵母细胞分别在三种不同培养环境中FSH→不含激素、FSH→LH、FSH+LH→不含激素。&→&

The purpose of this study is to establish an efficient way for mouse parthenogenetic activation.

为探讨一种高效的小鼠卵母细胞孤雌激活的方案，进一步提高孤雌囊胚发育率。

Effects of EGF on the in vitro maturation and parthenogenetic development of bovine oocytes were studied.

在卵母细胞体外成熟液中分别添加10，30，50ng/ml表皮生长因子，24h检查成熟率，并进行孤雌激活。

Parthenogenetic activation is artificially induced by various kinds of physical and/or chemical methods.

人工孤雌激活方法包括物理性激活和化学性激活以及联合激活方法，人卵母细胞的孤雌激活多以化学激活为主。

Our results showed that the parthenogenetic activation of mouse oocytes was affected by many factors, so the selection of optimum activated conditions was very important for the effect of activation.

实验表明，卵母细胞的孤雌激活受多种因素的影响，选择最佳的激活条件对孤雌激活效果尤为重要。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。