卵母细胞

Cell nucleus/transplantation ; electrofusion ; oocytes ; embryos

细胞核/移植；电融合；卵母细胞；胚胎

It is initiated by a mid-menstrual cycle release of luteinizing hormone from the pituitary and initiates a cascade of events that eventually lead to extrusion of a fertilizable oocyte as well as remodeling of the follicle into the corpus luteum.

这是一个发起中旬月经周期的黄体释放素由垂体和启动一连串的事件，最终导致挤出的卵母细胞如fertilizable以及重塑毛囊进入黄体。

From goldfish oocyte of maturation in vitro which was stimulated by 17α-20βpg (17α-hydroxy-20β-dihydroprogesterone), fertilizable ova and normally develeped juueniles were produced.

本研究主要用体外成熟的卵母细胞作为受体，将外源的美洲大绵鳚抗冻蛋白基因注入其生发泡，研究外源基因在金鱼中的整合和表达。

Oocytes from different size of follicular had different development ability.

不同直径大小卵泡中的卵母细胞体外成熟能力不同。

After 44 h IVM, MOS level in the immatured ooeytes was higher than matured oocytes, and there was less MOS in nucleus than in the cytoplasm at 6 h after matured oocytes were activated; RNAi was performed before IVM, and all the three siRNA applied could successfully knockdown the level of c-mos mRNA, which were 0.08 ± 0.03, 0.11 ± 0.06 and 0.20± 0.06 times of the control group respectively, and the quantity of MOS was obvious decreased compared with the control group at the same stage.

本实验通过RT- PCR、免疫荧光激光共聚焦检测方法检测了猪卵母细胞在体外成熟培养过程中c-mos基因在转录水平、翻译水平上的表达以及蛋白的分布，并应用注射小干扰RNA方法对其进行了RNA干扰研究。

With the great progress in embryo engineering during the past decade, the maturing oocytes with high qualities and quantities are increasedly required.

然而由于目前对卵泡发生和发育的调节机制仍知之甚少，因此大大制约了利用卵泡体外培养和体外成熟技术生产成熟卵母细胞的成功率。

The results demonstrated in this paper are good materials for furtherstudying the molecular mechanisms of oocyte meiotic resumption in vivo.

为进一步研究体内卵母细胞成熟调控的分子机制提供了很好的资料。

According to our knowledge, there have been a few reports on this field, but none was related to use meiotic inhibited agents for promoting oocyte developmental competence.

有关这方面的研究报道还不多，尤其在以核成熟抑制剂对卵母细胞产生抑制，并观察其后的胚胎发育活性方面。

Cycloheximide was proved to be a good inhibitors to block meiotic resumption of oocytes.

然而，CHX在山羊上的研究很少，对于CHX抑制后山羊卵母细胞的成熟、发育和胞质成熟都没有研究报道。

The in vitro meiotic process and developmental competence of buffalo oocytes from different size follicles was investigated.

探讨了不同大小水牛卵泡卵母细胞体外成熟过程中的减数分裂进程及其胚胎发育潜能。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。