卵母细胞

The oocyte is effectively a tetraploid cell.

卵母细胞是有效的四信体细胞。

AIM To study the isolation and in vitro culture of rabbit s bone mesenchymal stem cells and adipose stromal stem cells and the use of both BMSCs and ASSCs cells in nuclear transfer to produce totipotent blastocysts.

目的 研究非休眠期成年兔骨髓基质干细胞和脂肪基质干细胞作为供体，以体外成熟去核兔卵母细胞作为受体进行核移植构建重组胚胎的可行性及囊胚形成率。

The cloning of mammals by somatic cell nuclear transfer requires epigenetic reprogramming of the differentiated state of donor cell to a totipotent, embryonic ground state.

由于供体核支持整个克隆动物的发育，所以当供体核被移入去核的卵母细胞后，必需进行重编程，回到胚胎发育的起始阶段。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

Based on the experience and methods summed up by other researchers, the technique of cell culture was employed to study bovine follicular oocytes maturation, fertilization and the development of earlier embryo in vitro. The objective of this research is to upbuild and perfect a set of high-and-new-tech systems to obtain high-yield and high-quality bovine embryo, to provide technical operational regulations and ways and means to embryo engineering in practical production, to accumulate data for theoretical research.

本研究在总结前人研究的基础上，应用体外培养技术对牛卵母细胞体外成熟、体外受精及早期胚胎体外发育作了进一步研究，旨在建立健全一套完整的体外生产高产优质牛胚胎的高新技术体系，为胚胎工程和生产实践提供技术操作规程和方法，为理论研究积累资料。

The main point is to illustrate the effect of Uracil, ITS, and ATP on bovine oocytes'maturation.

旨在探讨成熟液中添加尿嘧啶和三价因子等试剂对牛卵母细胞成熟的影响。

And vitrification methods are more simple and convenient.

玻璃化冻存人类卵母细胞具有更大发展趋势。

Objective To explore the feasibility of vitrification of mouse maturation oocytes, using a close-carrier.

目的 探讨封闭式玻璃化冷冻载体冻存小鼠卵母细胞的可行性。

Objective To study the ultrastructural changes of wapiti oocytes and provide theoretical basis for wapiti breeding arid reproduction.

目的 通过对马鹿卵母细胞的超微结构进行研究，旨在为马鹿繁殖育种的研究提供基础理论依据。

Our studies on oocytes ultrastructure, in which experimental materials came from Wapiti, was just one of the blanks.

本研究以马鹿为试验材料，开展了鹿卵母细胞超微结构的研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。