卵母细胞

ABSTRACT 1. Effects of cycloheximide and cytochalasin B on the parthenogenetic development of buffalo oocytes were investigated.

系统探讨了放线菌酮和细胞松弛素B对水牛卵母细胞孤雌激活的影响。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

At this stage, the somatic cells are not endocrinologically active and thecal cells are not morphologically differentiated until follicles start to grow.

此时的初级卵母细胞由单层扁平前颗粒细胞围绕，形成始基卵泡。

The dynamic distribution of three different glycoconjugates in oocytes and follicle cells during the oogenesis of Atractomorpha sinensis were detected using biotin-labeled Peanut Agglutinin, Soy Bean Agglutinin and Ulex Europaeus Agglutinin I lectins.

以生物素标记的凝集素(UEA-I、 SBA、 PNA)为探针，利用凝集素组织化学方法对短额负蝗卵子发生过程中滤泡细胞和卵母细胞内糖复合物的分布进行了定位研究。

Studies on ultrastructure indicate that the follicular cells have the function to filter the extraneous substances, and they can also synthesize some vitellin materials toprovide nourishment which is needed by the development of the oocytc.

超微结构研究表明，滤泡上皮细胞除具筛选物质的作用外，还可合成某些卵黄物质，为卵母细胞的发育提供营养。

Mouse zygotic genome is activated at the two-cell stage which implies that embryonic development is transferred from the oocyte itself to the embryo.

在小鼠胚胎发育研究中发现，小鼠的基因组激活发生在2细胞期，这一时期标志着合子的发育由卵母细胞控制向胚胎控制的过渡，期间发生一系列复杂的生化过程。

The database searching result of spot M335 showed it might be splice isoform 2 of tropomyosin alpha 3 chain, splice isoform 2 of tropomyosin beta chain, or chain 1 of osteopontin.

成功地利用爪蟾卵母细胞和哺乳动物细胞的AQP1表达模型，从功能上证实碳酸酐酶抑制剂可抑制AQP1对水的转运。

In the present study, the immunocytochemical localization of ER of termite gonads were investigated with SABC method.

结果表明，ER在末龄若虫期的卵巢和精巢中都存在，免疫阳性物质定位在分化期和生长期的卵母细胞以及精原细胞中。

Methods The B6D2F1 hybrid mouse oocytes were parthenogenetic activated with strontium chloride(SrCI2) and cytochalasin B. The parthenogenetic blastocysts and morulas were seeded on MEF feeder respectively to examine the primary ICM appearance.and compare the derivation rate.

采用氯化锶联合细胞松弛素B激活B6D2F1杂交小鼠卵母细胞，所获得的囊胚与桑椹胚分别用于孤雌胚胎干细胞的建系，观察两者的建系成功率。

Particularly,the greatest damage of sharp was by using method D indicated that mouse oocytes and early embryos fixed in precooled methanol(-20℃) were inapplicable.

尤其以D方案对细胞形态的破坏最大，说明-20℃预冷甲醇不适用于小鼠卵母细胞和早期胚胎的固定。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。