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ABSTRACT Aim and Methods: Two-microelectrode voltage clamp method was used to study the blocking effects of extracellular Mn2+ on the inward rectifier pota ssium channel (IRK1) expressed in the Xenopus oocytes.

目的和方法:采用双微电极电压钳法研究细胞外Mn2+对非洲爪蟾卵母细胞表达的内向整流钾通道(IRK1)的阻断作用。

Aim and Methods: Two-microelectrode voltage clamp method was used to study the blocking effects of extracellular Mn2+ on the inward rectifier pota ssium channel (IRK1) expressed in the Xenopus oocytes.

目的和方法:采用双微电极电压钳法研究细胞外Mn2+对非洲爪蟾卵母细胞表达的内向整流钾通道(IRK1)的阻断作用。

The trail group which have all regulation elements of SCAP/SREBP pathway can express more fluorscence protein than the control group.[Conclusion] The experiment provides that the curcumin can upgrade the expression of low density lipoprotein receptor by SCAP/SREBP regulation pathway. The model can be applied for quick screening of the scap ligand drugs and inverstigating the mechanism of the drugs.

1下游基因的表达可能是姜黄素调脂作用的机理之一;利用核内共注射的方法在爪蟾卵母细胞中建立的SCAP/SREBP调控系统可作为SCAP配体类药物快速筛选和作用机制研究的细胞模型。

The preimplantation development competences of somatic cell nuclear transfer embryos reconstructed with enuleated goat Metaphase II oocytes matured in vivo and whole cells derived from adult fibroblasts of several mammalian species (goat, boer goat, bovine, tahr, panda) and human patient were evaluated.

研究去核山羊体内成熟的M II期卵母细胞与异种成年的哺乳动物(包括山羊、波尔山羊、牛、塔尔羊、熊猫)及人的成纤维细胞融合形成的体细胞核移植胚胎着床前的发育能力。

Dolly was the only lamb born from 277 fusions of oocytes with udder cells.

多利是277组卵母细胞和乳腺细胞的配对中唯一克隆成活的。

Immune POF model elicited by immunization in subcutaneous with antigen sourced isotype animal ovaries can cause growing follicles and mature follicles decrease obviously, the morphology of oocytes and zona pellucida abnormal, the adjacent granular cells disorder and atresic follicle increase.

以同种动物卵巢为抗原主动免疫建立的免疫性POF模型可致生长卵泡和成熟卵泡数明显减少,卵母细胞及透明带形态异常,周边颗粒细胞排列紊乱,闭锁卵泡增多,病理损伤随免疫时间延长而加重。

Result The BMP15 protein had no expression in oocytes of primary, secondary, antral follicles as well as granulosa cells and follicular flu. It was also detected in corpora lutea during early luteolysis at high level, but it was observed in atretic follicle at low level.

结果]BMP15蛋白在原始卵泡中没有表达,在初级卵泡、次级卵泡和有腔卵泡卵母细胞及有腔卵泡颗粒细胞和卵泡液中均有表达,在黄体溶解早期也有表达,但是在闭锁卵泡中的表达则受到抑制。

The translation activities of IL-2Ra mRNA among different kinds of cells by using Bufo bufo oocytes in vitro were studied.

本文采用中华大蟾蜍卵母细胞体外翻译系统研究了不同来源细胞IL—2RαmRNA的翻译活性。

The mammalian oocytes are important materials for study of meiotic cell cycle in developmental biology and cell biology.

哺乳动物卵母细胞是发育生物学和细胞生物学的重要研究对象。

In conclusion, our results showed that mPGES-2 might be involved in the processes of fertilization, oocyte/early embryo transport and early embryo development by catalyzing PGE2 synthesis in oviductal epithelium.

这些结果表明,mPGES-2 可能通过输卵管上皮细胞中合成的PGE_2,间接参与输卵管中进行的受精、卵母细胞和早期胚胎的运输,以及早期胚胎发育等过程。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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