卵母细胞

The results indicated that CIRP in the organisms could be induced at low temperature and might protect the organisms from the cold damage. The amino acid sequences deduced via cDNA clone were 100%, 99.4%, 95.5%, 67.4%, 58.4%,76.9%, and 79.1% identical to those of the CIRP in mice, rats, human, bullfrog and axolotl cells, and Xenopus embryos and oocytes, respectively.

根据克隆的cDNA所推测的氨基酸序列与GenBank上公布的小鼠、大鼠、人类、牛蛙、美西螈、非洲爪蟾胚胎细胞和卵母细胞的CIRP氨基酸序列同源性分别为100%、99.4%、95.5%、67.4%、58.4%、76.9%和79.1%。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

The appropriate electric fusion parameters for nuclear transfer of porcine somatic cells were investigated using in vitro matured porcine oosytes as recipients and granulose cells as donors in this study.

本研究用体外成熟42~46 h的猪卵母细胞为核移植受体，猪颗粒细胞为核移植供体，对猪体细胞核移植的电融合参数进行了探讨。

In recent years,the effect and regulation of microfilaments in the mature process of oocyte take more and more attentions.

微丝作为细胞骨架的组成部分，在卵母细胞成熟过程中的作用及调控近年来日益受到关注。

Because oocyte is a kind of specific cell in animal body, there are some unique phenomena and laws during its development.

因为卵母细胞是动物体内一种高度特化的细胞，在其生长发育过程中具有许多独特的现象和规律。

But there are many different viewpoints on the relationship between the apoptosis of granulosa cell and the oocyte developmental competence.

但是，关于颗粒细胞凋亡与卵母细胞发育能力之间关系的研究尚存许多矛盾。

The oocyte is one of the biggest and also the most difficult freezing cell type in the mammalian animal body.

卵母细胞是哺乳动物体内最大也是最难以冻存的一种细胞，其冷冻保存一直是生殖医学领域内研究的难点和热点。

The results showed that during oogenesis there was no distribution of the UEA-I receptor.

结果表明，在卵子发生的各期滤泡细胞和卵母细胞中没有UEA-I受体的表达， SBA和PNA受体以不同的分布模式呈阶段性表达。

Inseminated in 8-9 hrs after GVBD, the ova have the highest hatching rate (more than 86% in the concerned spawn).Based on the above insemination research of the oocyte maturation in vitro, recombinant opAFP-pUC19 was microinjected into the germinal vesicle of oocyte of maturation in vitro.

经过卵母细胞时期在生发泡中注射外源抗冻蛋白基因的体外成熟卵，其受精率（70～80%）和孵化率（38～75%）均低于对照组10～30%，在进行外源抗冻蛋白基因注射后授精孵出的小鱼长至半成体和成体鱼后，分别用斑点杂交和Southern blo-tting分析外源基因的整合。

The results demonstrated that the dup(p21) and the del(p11) were of paternal origin and i was of maternal origin. The dup(p21) arose from an unequal sister chromatid exchange. The del(p11) occurred through X chromosom e breakage and deletion machanism.

结果表明，dup(p21)和del(p11)起源于父方，而i起源于母方。dup(p21)是由X染色体姊妹染色单体不均等的互换所引起的，del(p 11)是由于X染色体断裂后丢失所致，i的发生是由于卵母细胞X染色体着丝粒错分裂。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。