卵母细胞

Vg could be detected in haemolmph of the water—fed female at 48h after eclosion, too.

用10％的蜂蜜和清水分别饲喂两组成虫(简称"蜜组"和"水组")，以研究成虫期的营养对Vg发生和卵巢发育的影响，结果显示，成虫期营养对于Vg的启动合成并不起关键的决定性作用，两组成虫均可以在羽化的48小时检测到Vg，且水组中的血淋巴水平并不低于蜜组对照，但绝大多数水组个体的卵巢发育停留在卵黄沉积的某一时期，此时在血淋巴中也检测不到，只有约20％的饲水个体卵巢可以发育成熟，出少量卵；用高剂量的ZR515于羽化当日点滴处理饲水个体，可以使所有接受处理的饲水个体的卵巢发育成熟，但是产卵〓依旧低于对照组的，不能恢复正常；这说明成虫期营养对卵巢中全体卵母细胞的成熟是必需的，其作用的产生是通过内分泌系统途径实现的。

In addition, many experimental designs in both research and clinical applications involve culture of matured oocytes prior to micromanipulation or insemination.

然而，人类和一些动物通常可以在发情周期的任何一天从事性行为，这可能导致排卵后老化的卵母细胞受精。

To observ the toxicity of infected mice and establish mice reproductive toxicity model;②To observe the infected BALB/c female mice ovulation quantums and ovum chromosome;③To observe the oestrogenic hormone( FSH, LH, E2 )level of infected BALB/c female mice;④To observe zygote developmental condition before nidation;⑤To observe embryonic development condition of infected mid-gravidity mice to research the reproductive toxicity and development toxicity of toxoplasma gondii to the BALB/c female mice.

目的： 本实验采用弓形虫感染BALB/c雌鼠，①观察染虫雌鼠的毒性反应，建立生殖毒性的小鼠模型；②染虫雌鼠排卵情况及卵母细胞染色体的变化；③染虫雌鼠性激素(FSH、LH、E2)水平的变化；④着床前受精卵的发育情况；⑤孕中期鼠染虫后胚胎的发育情况，探讨弓形虫对雌鼠生殖功能及胚胎发育的毒性作用。

This dissertation is divided into three parts. The first part is studies on calcium distribution changes during spermatogenesis, epididymal maturation, capacitation, acrosome reaction, oocyte maturation and egg fertilization using in situ precipitation by potassium antimonate and Ca〓 fluorescent probes at electron and light microscope levels. The main results are: 1 Ca〓 was probably involved in the condensing process of nucleoplasm during spermatogenesis.

本论文分为三个部分，第一部分主要是采用焦锑酸钾原位定钙法和Ca〓荧光染料在电镜和光镜水平对哺乳动物精子发生、附睾成熟、获能、顶体反应及卵母细胞成熟和卵受精过程中Ca〓的分布变化规律进行了系统的研究。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

Except for hemopoietic progenitor cells, spermatogenous cells and oocyts, there was no expression of hTERT in other somatic cells.

除造血祖细胞、精原细胞和卵母细胞表达外，其他正常体细胞中均不表达hTERT。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

Human fibroblast interferon mRNA was prepared from poly I:C-cycloheximide superinduced human diploid cells by phenol-octanol exhaustion method followed by oligo-cellulose chromatography.

其主要实验根据是：超诱导细胞mRNA含量明显高于正常细胞；干扰素mRNA在非洲鲫鱼卵母细胞内转译的产物具有明显的抗病毒活性；转译时~14C-苯丙氨酸的掺入明显高于正常细胞；种属特异性实验及酶的敏感性实验证明转译产物中存在的抗病毒物质是一种人的干扰素蛋白质。

At the same time,oocytes and spermatids were used as the samples to check the sensitivity of this nested PCR The results showed that this method can successfully check the templates of ten cells.

同时，以微量的卵母细胞和精子细胞为模板对该反应体系的灵敏性进行了检验，结果表明，该反应体系可对10个细胞水平进行准确鉴定。

DNA fluorescence dye Hoechest 33342 was used tomonitor the condensation of chromatin during germinal vesicle breakdown.

采用直接取核法获取卵母细胞生发泡，用同步化的HeLa细胞裂解液构建非细胞体系，将游离的生发泡培养在裂解液中，并加入荧光染料Hoechest 33342，观察染色质的变化情况。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。