卵母细胞

Studies on mouse nuclear transfer with compacted morula blastomeres as donors In this study, C57BL/6 mouse morulae blastomeres and Kunming white mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different sucrose concentration on the manipulation time of nuclear transfer, and the in vitro development of reconstructed embryos.

以致密桑椹胚卵裂球为供体克隆小鼠的研究该研究以C57BL/6 小鼠致密桑椹胚卵裂球为供体、昆明白小鼠MⅡ期卵母细胞为受体，研究了不同蔗糖浓度对核移植操作时间及重构胚体外发育的影响。

Do not cause decomposition of one ovulation, mature follicles to a certain stage, clearly highlighted in the ovarian surface, with the proliferation of follicular fluid, internal pressure increases, so that more and more prominent part of the ovarian tissue is thin, the final rupture secondary oocyte cells and their peripheral zona pellucida and corona radiata, together with the discharge of ovarian follicular fluid in the process called ovulation.

不排卵原因分解1、成熟卵泡发育到一定阶段，明显地突出于卵巢表面，随着卵泡液的激增，内压的升高，使突出部分的卵巢组织愈来愈薄，最后破裂次级卵母细胞及其外周的透明带和放射冠随卵泡液一起排出卵巢这一过程称排卵。

The sperm subzonal insemination on mice denudedoocytes. This part of paper use SUZI method to investigate the effects of zona pellucidaon denuded oocytesfertilization.

二、精子注入去除卵丘细胞的小鼠卵母细胞的体外受精利用透明带下精子注射技术，研究透明带对受精的影响。

Embryo cells display a synchron cleavage rhythm during the cleavage stage, there is almost no gene transactivation, which is regulate and operated by the mRNAs and protein of oocyte.

卵裂阶段的胚胎细胞呈现同步化的分裂节律，鲜有基因转录活动，其发育由源自卵母细胞发生期间合成的蛋白质和mRNAs调控。

The results indicated that the receptors of estrogen, androgen and progestogen existed in oogonia and oocytes of different developmental stages.

结果表明，不同发育时期的卵原细胞和卵母细胞中都存在雌激素、雄激素和孕激素受体。

The results showed that Golgi complex was an organelle that experienced a series of changes during four stages of the oogenesis of Bullacta exarata , which were oogonia, early vitellogenic oocyte, mid-vitellogenic oocyte and end vitellogenic oocyte.

结果表明，在泥螺卵子发生的四个时期即卵原细胞期、卵黄发生早期、卵黄发生中期和卵黄发生后期，卵母细胞内高尔基复合体经历了一系列的变化。

GIFT is again the removal of the oocyte from the ovary, but instead of fertilization outside the body, the egg and sperm are mixed together, placed into the fallopian tube, where fertilization and embryo development take place (this is where the sperm and egg normally meet).

GIFT也是从卵巢取出卵母细胞，但是并不是在体外进行受精，而是将卵和精子混合在一起，送回到输卵管，受精和胚胎发育是在那里进行的（这就是正常情况下卵和精子相遇的地方）。

Methods and Results Experiment 1: Intracytoplasmic injection of rat sperm heads was performed using 7~10 μm and 2~4 μm diameter pipettes and different injected method respectively.

方法和结果实验1：用直径为7~10μm和2~4μm的注射针以及相应的注射方法进行大鼠ICSI，ICSI后卵母细胞存活率(30.5% vs。 61.3%)和卵裂率(12.5% vs。 51.1%)均差异显著(P.05)；实验2：分别在hCG后14 h、16 h和18 h取卵进行ICSI，三组存活率77.4%、74.1% vs。

4H was the best time of bovine oocyte activationin the 6-DAMP, and the cleavage rates and blastula rates could reach 85.1% and 26.8%. 4. IVF-100 was used in vitro fertilization, because IVF-100 was not needed to prepare usually,store conveniently, and gain a higher cleavage rates. After the oocytes have matured for 20h, theywere digested by permease and treated with suctionpipe.

由于IVF-100无需经常配制、保存方便且可以获得较高的卵裂率，所以本实验采用IVF-100为受精液，卵母细胞成熟20小时后采用透酶+口吸管消化的方法处理，获得了较好的受精效果，卵裂率可达(76.8％)。

Aims and methods: To investigate the distribution of oncoprotiem, ErbB2 in mouse testis, epididymidis, ovaries, oviducts, oocytes-cumulus cells complex and sperms, immunobistochemical method was used.

目的和方法：用免疫组织化学的方法观察ErbB受体家族成员ErbB2在小鼠睾丸、附睾、卵巢、输卵管、卵母细胞—卵丘细胞复合物和精子上的分布。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。