卵母细胞

However, the exact mechanism by which cumulus cells promote oocyte aging is unknown.

我们之前的研究证明卵丘细胞能够促进小鼠卵母细胞老化。

To evaluate the role of mature cumulus cells from oocyte-cumulus complex in in-vitro maturation and establish a new culture technique which is convenient to carry out.

探讨人类成熟卵丘细胞在未成熟卵母细胞体外成熟培养中的作用，并建立一种简易的实施技术。

Denude oocytes matured in vitro have a clearly deficiency on fertilization invitro.

3去除卵丘细胞的小鼠卵母细胞在受精时存在着明显的障碍。

At the small growth stage, three kinds of the sex steroid receptors usually were localized in the cytoplasm or nuclear membrane of oogonia and early primary oocyte, and localized in the cytoplasm and nucleoplasm of oocytes at the large growth stage and mature stage.

在小生长期，3种受体通常定位在卵原细胞和早期初级卵母细胞的胞质或核膜；在大生长期和成熟期，则定位在胞质和核质。

The regression analysis shows that the development of the oogonium is accelerated at low temperature and longer daylength (from Dec. to Mar.).

回归分析显示，低温和延长的日照时间（12~3月）促进卵原细胞增殖；高温和长日照（4~6月）促进卵母细胞生长。

These results indicated that CB is necessary for synchronization in spindle formation in both oocyte and primary spermatocyte, and that synchronization may be required for subsequent embryo development.

这一结果表明CB的处理可以使精母细胞与卵母细胞同步形成MI纺锤体，使其发育同步，这可能对以后胚胎的发育具有重要作用。

Silence of AbdB gene led to abnormality of oviduct and gathering of unfledged eggs at the end of the oviduct.

未成熟卵中的卵母细胞和滋养细胞也发育异常，呈空泡状和碎片状。

Results Under the light microscope, we had figured out that the mean diameter of wapiti oocyte was 172.958±17.298 μm, and the thickness of cumulus cell and zona pellucida were 67.750±33.107 μm and 13.782±2.516μm respectively.

结果 在光镜下，马鹿卵母细胞的平均直径为172.955±17.295μm，卵丘细胞厚度为67.750±33.107μm，透明带厚度为13.782±2.516μm。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

A single donor cell was injected into the perivitelline space of enucleated oocyte and fusion was induced with electric pulses. Reconstructed oocytes were activated using ionomycin (5 M,5min) and 6-dimethylaminopurine (6-DMAP,2mM,5h).

将供核细胞注射到去核卵母细胞的卵周隙，经电融合获得重构胚，再经离子霉素(Ionomycin，5μM，5min)和6-二甲基氨基嘌呤(6-DMAP，2mM，5h)处理激活。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。