卵母细胞

The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

试验获得了如下结果：1。以山羊耳成纤维细胞为供体，以牛、羊、兔卵母细胞做为胞质受体构建重构胚，并经体外培养，发现不同的胞质受体均能支持重构胚的早期发育，但受体胞质对重构卵的重构胚早期发育速度和卵裂率均有显著影响。

The results show that the CD29, CD49, CD90 express in the gonium and the primary spermatogenous, but there are not expression in the oocyte, the second spermatogenous and the spermatocyte.

研究结果表明，CD29， CD49， CD90在雌性生殖腺各级卵母细胞中均不表达，位于生殖褶边缘的性原细胞中可见表达；在雄性生殖腺的初级精原细胞中表达，但在成团分布的次级精原细胞、各级精母细胞中均未表达。

Because the signaling molecules RAS and ERK1/2 (extracellular signal–regulated kinases 1 and 2) are activated by an LH surge in granulosa cells of preovulatory follicles, we disrupted Erk1/2 in mouse granulosa cells and provide in vivo evidence that these kinases are necessary for LH-induced oocyte resumption of meiosis, ovulation, and luteinization.

研究人员仍然在试图解析接着发生的那些导致排卵（或在某些情况下没有排卵）的分子信号级联反应。研究人员现在证明，激酶ERK1和ERK2是该激素信号的至关重要的下游标靶。在那些颗粒细胞中缺乏这些酶的雌性小鼠中，其卵母细胞不会成熟，因而不会从它们的滤泡中释放出来。

A new method for enucleating MⅡ nucleus in mouse and rat was induced; Living births were obtained from IVF of mouse oocytes reconstructed by transfer of metaphase Ⅱ chromosomes. For the interspecies nuclear transfer between mouse and rabbit, the reconstructed embryos could develop into blasocyst in vitro.

在不同品系小鼠卵母细胞核质交换移植中将电融合的重构卵进行IVF，经胚胎移植后顺利产仔；在异种核移植方面，小鼠核与兔卵母细胞的异种重构胚在体外培养条件下发育至囊胚阶段。

Previous study has shown that roscovitine did not inhibit germinal vesicle breakdown of goat oocytes until the concentration increased to a high dose.

之前的研究证明，高浓度的ROS能抑制山羊卵母细胞发生GVBD，但长时间高浓度抑制损伤卵母细胞的发育能力。

Objective To determine whether 18S rRNA is expressed in ovaries and single immature oocytes in germinal vesicle and metaphaseⅠ or not and reveal the relationship between 18S rRNA and maturation of oocytes.

目的检测小鼠18S rRNA基因在卵巢及单个GV期、MⅠ期卵母细胞中的表达，揭示核糖体18S rRNA基因与小鼠卵母细胞成熟之间的关系。

LH plaies a role to delay the occurrence of germinal vesicle, prolong the maturation duration of cytoplasm, enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro, and finally improve the potentiality of their in vitro development.

LH可能通过延迟生发泡破裂和促进胞质成熟的方式，使绵羊卵母细胞的核质成熟趋于同步，有助于绵羊卵母细胞的体外成熟以及受精后的进一步发育。

In one group oocytes were cultured for 3h and in the other one for 6h. Incidence of germinal vesicle breakdown was observed following the culture with BL-Ⅰ.

BL-Ⅰ培养液培养结束时，观察卵母细胞生发泡破裂的发生率，再用不含BL-Ⅰ的培养液继续培养，进一步观察其成熟动力学的变化，即卵母细胞GVBD过程和第一极体形成过程。

PKA activators such as cAMP, 8 - Br -cAMP or phosphodiesterase inhibitor isomethyl butyl xanthine or purified PKA catalytic subunit all can inhibit germinal vesicle breakdown and meiotic maturation in mouse oocytes, also in Xenopus oocytes.

以小鼠卵母细胞为实验对象，给予PKA激动剂cAMP、8-Br-cAMP或磷酸二酯酶抑制剂IMBX均可抑制小鼠卵母细胞的胚泡破裂及减数分裂成熟。

In this study, we investigate the cryobiology character of rabbit and human oocytes inorder to find a better freezing method, which can improve the survival rate and the following embryo development. We hope this study can provide some laboratory proofs for the future use of this technique.

本研究旨在探讨卵母细胞的冷冻生物学特性，寻找更好的冷冻方法进一步提高存活率，减少解冻后卵母细胞及其所形成胚胎的损伤，建立可行的、冷冻后存活率及发育率较高的方案，为此技术最终应用于临床提供一定的实验室依据。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。