卵母细胞

Our study demonstrated that the use of 3% sucrose can give a judgment to embryo quality and then ooctyes with good quality could be selected and enucleated by this method for further micromanipulation.

所以，3％的蔗糖处理还可用于卵母细胞质量好坏的鉴别，有利于在较短时间内挑选出好的卵母细胞进行去核或进一步的操作。

In order to find an effective nuclear transfer program, this thesis investigated three factors affecting the efficiency of somatic nuclear transfer on goats: the in vivo mature time of oocytes, the component of operation liquid for oocyctes enucleating and the parthenoactivation mode of oocytes.

本实验通过改变以下试验条件：卵母细胞体外成熟时间的不同、卵母细胞去核时操作液成分的不同，来研究其对山羊核移植效率的影响，希冀寻找一个有效的核移植方案。

Enucleating the nuclei of recipient oocytes accurately is one of the key techniques in nuclear transfer research. Enucleation of mouse MⅡoocytes can be performed under an inverted microscope equipped with Normarski or Hoffmann optics.

准确去除受体卵母细胞的细胞核是核移植研究的关键技术之一，在装备有Normarski 或Hoffmann 光学镜头的倒置显微镜下可以准确去除小鼠MⅡ期卵母细胞的细胞核。

Although the technology of In Vitro Maturation is more and more maturated, the mechanism of maturation needs further study.

虽然牛卵母细胞体外成熟培养技术已趋于成熟和完善，但卵母细胞成熟机理的研究还有待深入。

Expression of tert and hprt genes were detected simultaneous in all single-copy embryos developed at 4-C (65h-post hCG, 4/4), 8-C (75 h-post hCG, 4/4), morulae (93h-post hCG, 4/4) and blastocyst (118h-post hCG, 4/4) stages and the level of transcription increased.

结果表明，持家基因hprt在成熟卵母细胞受精前后，以及胚胎早期发育过程中均存在转录本。40%卵母细胞中存在的tert mRNA在受精后很快降解，检测不到；胚胎基因组在2-C早期开始转录tert mRNA，转录水平逐渐上升。

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

It was discussed on effect of oocytes maturation and fertilization in vitro of Nanyang cattle by different collecting ways.

探讨了卵母细胞的不同采集方法对南阳牛卵母细胞体外成熟的影响。

And he relationship between ovarian artery blood flow and the number and quality of oocytes retrieved.

分析回收卵母细胞数和成熟卵母细胞数与卵巢动脉血流之间的关系。

It is necessary to develop a protein-free media for oocytes maturation that can prevent zona hardening. In this study, immature macaque oocytes were cultured in modified CMRL media alone or CMRL with either bovine calf serum or PVA for 24 hours. Also, in vivo matured oocytes were cultured in CMRL supplemented with PVA or serum for approximately 6 hours.

我们在本研究中，将非成熟的猕猴卵母细胞分别在改良的CMRL，或在含有胎牛血清或聚乙烯醇的改良CMRL培养基中培养24小时；同时，将体内成熟的卵母细胞分别在含有PVA或者胎牛血清的CMRL中培养6小时，然后进行体外授精，比较各组间精子穿透率或受精，以及胚胎发育情况。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。