卵母细胞

AIM: To explore optimal superovulating methods and parthenogenetic activation conditions in rabbits.

目的：探索兔卵母细胞的最佳超排方法及孤雌激活的条件，为人兔异种核移植提供充足的兔卵母细胞。

A23187 and OA could parthenogenetically activate Urechis unicinctus oocytes and activate MAPK, indicating that the effect of fertilization on GVBD and activation of MAPK was mediated by rising in intracellular free calcium, and phosphorylation of some proteins was enough to activate the oocytes.

钙离子载体A23187和OA可以孤雌活化单环刺螠卵母细胞并激活MAPK。说明：①受精是通过钙离子的内流来激活卵母细胞并使MAPK活化的。②某些蛋白质的磷酸化状态对减数分裂是必要而且在一定程度上是充分的。

This antibody cross-reacted with the Vasa signals in the perinuclear nuage-like structures of young oocytes and some oocytes bearing early and mid-vitellogenesis.

实验结果显示在刚分化的卵母细胞以及在卵黄生成早、中期的卵母细胞，较集中的Vasa讯号出现在核膜周围。

In oocytes matured in vitro for 48h, polyspermy ratio is 73%, and lower than that in 36h(83%)and 24h(80%).

结果显示，体外成熟48h的卵母细胞其多精受精率为73%，比体外成熟36h(83%)、24h(80%)的卵母细胞要低。

Intracytoplasmic sperm injection, which is a new technique of embryo engineering of domestic animals, can solve the problem of porcine polyspermy.

用GFP基因转染的精子注射后化学激活的ICSI卵母细胞基因表达率显著高于电激活处理的ICSI卵母细胞的基因表达率。

The cleavage and pronuclear formation rate of oocytes treated with 5μmol/L Ion were significantly higher than that of oocytes with EH treatment.

结果发现，5μmol／L Ion激活处理水牛卵母细胞分裂率（63.0％vs 54.2％，P＜0.05）及原核形成率显著高于EH处理的卵母细胞。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后，分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h，研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

Most of EGFR in unmatured and matured oocytes was in variation, and there was obviously more expression of variation in matured oocytes than that in unmatured oocytes.

未成熟和成熟的卵母细胞中主要存在Ⅷ突变型，而野生型表达相对较少，成熟后卵母细胞中的Ⅷ突变型蛋白明显增加。

During zebrafish oogensis, vasa mRNA is expressed strongly and uniformly distributed in the cytoplasm in stage Ⅱ oocytes, followed by a distribution among vacuome in stage Ⅲ.

随着卵母细胞的生长，第Ⅲ期卵母细胞中的vasa mRNA分布在液泡之间。

In order to establish mouse nuclei transfer technology, mouse zygote culture medium and activated conditions were optimized in vitro and enucleated method of MII oocyte were investigated in this study.

本研究通过对小鼠受精卵体外培养体系，MII期卵母细胞孤雌激活条件的筛选以及探讨MII期卵母细胞的去核方法为小鼠体细胞核移植的成功奠定基础。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。