卵母细胞

The results indicate that chromium trichloride may hazard the meiotic maturation of mouse oocyte resulting in reducing the ability of fertilization and may be toxic to women′s oocyte and reproductive capacity.

结果提示，三氯化铬可以破坏卵母细胞的成熟，降低卵母细胞的受精能力，具有明显的生殖毒性。

In order to investigate the biophysical characteristics of porcine oocytes, mechanics property of the zona pellucida of porcine oocytes was studied.

［目的］为了探讨猪卵母细胞的生物物理特性，该文研究了猪卵母细胞透明带的力学性质。

However, zona hardening could occur during in vitro maturation of oocytes in a simply defined medium which reduced the fertilization rate.

采用无蛋白培养基对卵母细胞进行体外成熟培养，并防止透明带硬化，是研究影响卵母细胞成熟过程因素的基础。

At various times of culture oocytes were stained and examined by confocal microscopy.

在培养的不同阶段对卵母细胞进行免疫荧光染色发现CB处理与否对卵母细胞的发育有重要影响。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Objective To clarify the relationship between oocyte maturation and mitochondria distribution by investigating the distribution of mitochondrial in immatured and matured human oocytes.

目的研究人未成熟和成熟卵母细胞中线粒体的分布，进一步阐明卵母细胞成熟和线粒体分布的关系。

Methods Both immatured and matured oocytes were stained by Mito Tracker Green FM.

方法未成熟卵母细胞和成熟卵母细胞均用Mito Tracker Green FM染色，经多聚甲醛固定后在激光扫描共焦显微镜下观察线粒体的分布。

The migration of meiotic spindles to the cortex is accompanied by a local reorganization and polarization of the cortex.

本论文研究进化上保守的Pins同源物在小鼠卵母细胞中的功能。 LGN是在小鼠卵母细胞中表达的Pins同源物。

The results indicated that meiotic inhibition in vitro could be used as a tool in the study of the mechanisms involved in acquisition of meiotic and embryonic competence.

因此，卵母细胞成熟的可逆性抑制可用于研究卵母细胞成熟及其胚胎发育活性的机制。

Follicular oocytes had developed to the stage of GV, GVBD, M I, MII after 0 h, 4 h, 14 h and 24 h of culture, respectively, judged by morphological cretirea of oocyte chromosome stained with orcein.

根据地衣红染色，对山羊卵母细胞的核染色体的形态观察发现，直径为2-3mm的卵泡其卵母细胞在体外分别培养0、4、14和24小时即可发育到GV、GVBD、MⅠ、MⅡ期。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。