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I have constructed and screened a cDNA library of Xenopus oocytes with E-cadherin probe. One of the four cadherin-related mRNAs analyzed is possibly localized to the animal pole.

在本篇论文中,我们建立了蛙卵母细胞的cDNA library,并利用E-cadherin 探针从中选殖到几个cadherin-related基因,在已分析的四个基因中有一个基因在卵母细胞中有不均匀分布发生。

After the onset of meiosis reinitiation, the GV stated to migrate along microtubules to the animal pole and attached to the site beneath oocyte membrane, that followed by GVBD.

通过激光共聚焦显微镜观察,未成熟的卵母细胞中存在5种类型的微管,并且有两个微管组织中心锚定在卵母细胞突起下方。

Our data support the notion that human follicle development can be achieved in vitro in a bio-engineered culture system.

结论我们的数据表明,人类卵泡在体外生物工程培养体系中是可以发育的,但仍需要更多的研究进一步揭示体外培养卵泡是否获得充分大小的卵母细胞,而且卵母细胞是否有能力恢复减数分裂和受精。

Methods: Human mature oocytes were cryopreserved using cryoleaf. Resuscitated oocytes were administered by intracytoplasmic sperm injection, embryo transplantation or cultured to blastulation, observing the fertilization and developmental capacity of oocytes.

运用cryoleaf进行人类成熟卵母细胞玻璃化冷冻保存,复苏后行胞浆内单精子注射-胚胎移植或培养至囊胚形成,观察复苏后卵母细胞受精及发育能力。

Although the oocytes collected from ovaries before IVM are usually arrested at the diplotene stage, they are different in developmental competence.

用于IVM的卵母细胞虽然都停滞在第一次减数分裂的双线期,但质量是不同的,造成卵母细胞体外成熟结果有很大差异。

Enucleation by Spindle-View system could eliminate the effects of changing of the position on enucleation rate.

结果发现,随着卵母细胞成熟时间的延长,卵母细胞的核与PB〓的相对距离增大。

After being dispersed by simply beat upon with syringe and adherent culture, the mature cumulus cells from mature OCCs in COH cycles, together with growth factors in the follicular fluid or extraneously supplemented, could promote the IVM of immature oocyte.

来自控制性促排卵周期的成熟卵丘细胞经简易吹打分散后贴壁培养,可能能协同卵泡液中或外加的生长因子,促进未成熟卵母细胞的体外成熟,而本研究技术简易有效,可用于挽救促排卵周期的未成熟卵。

The Ca〓 oscillations occured before GVBD were associated with the resumption of GVBD, because when the oocyte was cultured in medium with hypoxanthine, no Ca〓 oscillation occurred, without hypoxanthine the oscillations occured again.

这些Ca〓波动与卵母细胞的GVBD有关,被次黄嘌呤抑制于GV阶段的卵母细胞不出现这种形式的波动,当再次培养于无次黄嘌呤的培养液中则又出现Ca〓的波动。

In this paper the effects of mercury chloride on the coefficient of visceral organ of mouse and the meiotic maturation and the fertilization ability of mouse oocyte were studied by in vitro culture and in vitro fertilization of mouse oocytes,etc.

为探讨汞化合物对脏器及生殖的毒性,采用卵母细胞体外培养、体外受精的方法研究了汞对小鼠脏器系数、卵母细胞成熟和受精能力的影响。

The results indicated that mercury chloride could affect the meiotic maturation of mouse oocyte,obviously block the IVF and injury or make a reduction of reproductive capacity of mouse.

结果提示,氯化汞可以影响卵母细胞的成熟,明显破坏卵母细胞的体外受精能力,损伤或降低小鼠的生殖能力。

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