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Methods SP immunohistochemical staining was used to detect the level of VEGF and EG-VEGF in 85 cases of the epithelial ovarian tumor tissue(25 cases of benign tumor,27 cases of ovarian carcinoma of stage FIGOⅠ、Ⅱ,33 cases of ovarian carcinoma of stage FIGOⅢ、Ⅳ)and 20 cases of normal ovarian tissue.The integral optical density was measured.

方法应用免疫组织化学SP法和形态定量学方法检测85例卵巢上皮性肿瘤(良性25例,FIGOⅠ、Ⅱ期卵巢癌27例,FIGOⅢ、Ⅳ期卵巢癌33例)和20例正常卵巢(除外卵巢疾病的绝经前患者)组织中VEGF、EG-VEGF表达的阳性率及积分光密度值。

Objective To explore the value of laparoscopic operation in the treatment of gynecologic diseases.Methods Retrospective analysis of 170 cases of laparoscopic surgery in ynecology.53 cases in the salpingocyesis group,49 cases salpingotomy,4 cases salpingectomy;48 cases in the ovarian cyst group,35 cases enucleation and repair of ovary,8 cases ovary sutures atches,3 adnexnectomy and 2 perforation of ovary;52 cases in the uterine problems group,10 cases LM5 cases CISH,11 cases LSH(laparoscopic supracervical ysterectomy),14 cases LTH(laparoscopic total hysterecotomy),12 cases LAVH laparoscopically assisted vaginal hysterectomy);17 cases in the infertility group were studied in terms of lysis of pelvic adhesions and salpingostomy.

目的 探讨妇科腹腔镜手术的临床应用价值方法回顾性分析170例妇科腹腔镜手术病例,其中异位妊娠组53例,行输卵管切开取胚49例,输卵管切除术4例;卵巢疾患组48例,囊肿剥除术及卵巢成形术35例,卵巢缝合修补8例,患侧附件切除3例,囊卵巢多点打孔2例;子宫疾病组52例,行肌瘤剥除10例,改良CISH术5例,子宫次全切除术11例,子宫全切术14例,腹腔镜辅助阴式子宫切除12例;不孕症组17例,行盆腔粘连分解输卵管伞造口术。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术,建立了对虾二倍体和三倍体卵巢间的2个消减文库;在正向消减文库(以三倍体卵巢作为实验组,二倍体卵巢作为驱动组)中,鉴定到54个基因;在反向消减文库(以二倍体卵巢为实验组,三倍体卵巢为驱动组)中,鉴定到16个基因;选取11个差异表达的基因,利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测,均能很好地与消减结果相吻合;这些差异基因编码多种功能的蛋白,分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响;为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

Methods Fetal ovaries were transplanted to rats who had received unilateral ovarian extirpation or oophorotomy, and then the interaction between local ovaries and transplanted ovaries was detected.

将胚鼠卵巢分别移植至单侧卵巢摘除及去势雌鼠皮下,观察同时存在的自身卵巢对移植卵巢生长发育有无影响,以及自身卵巢是否受移植卵巢影响。

The positve rates in serous carcinomas and borderline tumors were 39.3% and 55.6%, significantly lower than that in benign tumors and normal ovaries (P.05), but the statistical differences were not found between borderline tumors and serous carcinomas, benign tumors and normal ovaries.2 WWOX mRNA and protein expression in ovarin seruous tumors2.1 WWOX mRNA expression in ovarin seruous tumors The result of RT-PCR showed that WWOX mRNA expression were detected in 29 of 32 ovarian serous benign tumors, 12 of 16 ovarian serous borderline tumors, and 21 of 45 ovarian serous carcinomas respectively.

阳性条带密度扫描定量结果,卵巢浆液性癌中PTEN蛋白表达相对量为0.34±0.12比正常卵巢和浆液性囊腺瘤和交界性囊腺瘤明显降低(p.05),其余各组间无统计学差异。3.2.2 PTEN蛋白FCM检测结果FCM结果,卵巢浆液性癌PTEN蛋白表达的FI值(0.908±0.023)明显低于正常卵巢、卵巢囊腺瘤和交界性囊腺瘤的FI值,差异有统计学意义(P.05)。3.2.3 PTEN蛋白免疫组织化学结果35例浆液性囊腺瘤、18例交界性囊腺瘤和56例浆液性癌中,分别有31例、15例和29例呈阳性表达。

Methods Expression of p16 was examined in 30 cases of normal ovarium, 32 benign ovarian tumor, 8 borderline ovarian tumor and 103 ovarian cancer by using ABC immunohistochemistry. The association between p16 expression and clinicopathology was also analyzed.

用免疫组化 ABC法检测30例正常卵巢、32例卵巢良性肿瘤、8例交界性卵巢肿瘤和 10 3例卵巢癌组织中的抑癌基因 p16蛋白表达情况,分析 p16蛋白表达与卵巢癌分级分期、转移预后的关系。

Methods UGBOABF are measured in 60 women undergoing IVF treatment by transvaginal color Doppler on the second day of the stimulating cycle,and the same time perifollicular numbers and ovarian volume are measured by B ultrasound and to detect FSH basal of solution.

经阴道彩色多普勒探头观测63例接受体外受精-胚胎移植治疗的妇女月经第2天卵巢动脉血流指标,预测卵巢储备功能;同时超声测量窦卵泡数+卵巢体积+血FSH值,预测卵巢储备功能;比较两种方法是否存在差异。

Methods UGBOABF are measured in 60 women undergoing IVF treatment by transvaginal color Doppler on the second day of the stimulating cycle,and the same time perifollicular numbers and ovarian volume are measured by B ultrasound and to detect FSH basal of solution.

体例 经阴道彩色多普勒探头不雅观察63例担任体外受精-胚胎移植治疗的妇女月经第2天卵巢动脉血流指标,展望卵巢储备功效;同时超声丈量窦卵泡数+卵巢体积+血FSH值,展望卵巢储备功效;斗劲两种体例是否存在差别。

Methods: Fifty-four female SD rats (8-month-old) were randomly divided into three groups, 18 rats in each group, They included a sham-operated group, an ovariectomized group and a tonifying kidney recipes therapy group, After 3 months, the animal models of osteoporosis were established, pour titanium smooth columnar implants were placed in the proximal metaphyses of left tibiae of the rats.

选用纯种8月龄雌性SD大鼠54只,随机分为3组:假手术组,卵巢切除组,卵巢切除加中药治疗组,每组18只。切除卵巢切除组及中药组大鼠双侧卵巢,假手术组大鼠仅切除双侧卵巢附近的脂肪组织。术后3个月,在三组大鼠胫骨近中干骺端分别植入纯钛光滑柱状种植体。

The snails used for this study, measuring within the range of 7.1 to 3.0mm., were collected from the banks of Ching-San River, Chungking, Kiangsu.

从11月到第二年5月,卵巢呈丰满而多卵的状态;6月卵巢趋向萎缩;7、8月卵巢萎缩,内仅含有少量的幼稚卵细胞;9月卵巢开始趋向丰满;10月卵巢逐渐恢复丰满的状态。卵巢全年周期变化的过程与镇江地区钉螺的产卵情况基本上相符合。

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