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Methods] A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法] 采用基因芯片技术,按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA;逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针,在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods: The expression of p16 protein and nm 23 protein were detected by an immunohistochemistory assay in 130 cases of tissue samples including 30 normal ovary, 50 ovarian benign epithelioma and 50 ovarian epithelial carcinoma.

免疫组化SP法检测30例正常卵巢,50例卵巢上皮性良性肿瘤及50例卵巢癌组织中P16蛋白和nm23蛋白,并分析其阳性表达与肿瘤的关系。

Methods: The expression of P16 protein and nm23 protein were detected by streptavidin peroxidase conjugated method in 78 cases of tissue samples including 12 normal ovary,14 ovarian benign epithelioma and 52 OEC.

免疫组化S P法检测12 例正常卵巢,14 例卵巢上皮性良性肿瘤及52 例卵巢癌组织中 P16 蛋白和nm 23 蛋白的表达。

It was the first time that using 〓 cell line and normal immunity inbreed line BalB/c mice as objects to construct the subcutaneous transplanting ovarian cancer model. Histopathological experiment proved that the transplanting tumor was undifferentiated cancer. The ovarian tumor marker 〓 and the epithelial tumor marker 〓 were positive in the transplanting tumors. According to these proofs we thought we had already succeeded in constructing animal tumor model.

本实验首次采用卵巢癌〓细胞系,以正常免疫力的雌性近交系BalB/c小鼠为对象,建立了卵巢癌皮下移植瘤模型,经病理组织学检查证实移植瘤为低分化癌,经免疫组化检测证实移植瘤组织中卵巢肿瘤标志物〓和上皮性肿瘤标志物〓表达阳性,据此我们认为动物肿瘤模型建立成功。

We detected the expression of OCY-66, OCY-189 and OCY-142 in 28 ovarian cancers and 30 normal ovarian tissues by RT-PCR, and studied the expression of OCY-142 in these ovarian cancers and normal ovarian tissues by nonradioactive hybridization in situ.

采用RT-PCR和原位杂交的方法研究卵巢癌相关肿瘤抗原新基因在卵巢癌组织和正常卵巢组织中的表达,分析其与卵巢癌临床指标的关系。

The estimated cumulativeincidence of peritoneal cancer is 4.3% at 20 years after oophorectomy.

研究者观察,在卵巢完全无损伤的妇女只有一个腹膜癌案例,在卵巢切除术妇女有7个案例;卵巢切除术后20年,估计的腹膜癌累积发生率是 4.3%。

Objective To study the clinical characteristics of clear cell carcinoma of the ovary.

卵巢透明细胞癌是卵巢上皮性癌的一种,约占卵巢上皮性癌的 5 %~ 11% [1] 。

Objective To establish rat model with polycystic ovary with two different methods:testosterone plus human chorionic gonadotropin and progestogen plus HCG,compare the rat models induced by testosterone plus HCG with the patients with polycystic ovary syndrome in the aspects of ovarial morphous and gonadal hormone,and investigate whether testosterone with HCG is the effective method to establish rat model with polycystic ovary syndrome,than compare two methods(T+HCG and P+HCG)finally to draw a conclusion if there are any difference or similary between the two methods and which one is more ideal and convenient.Methods 60 SD female rats of 23 days were divided into three groups in random.

目的 本实验用睾酮联合绒毛膜促性腺激素及炔诺孕酮联合HCG两种方法分别建立多囊卵巢大鼠模型,将模型鼠的卵巢形态及血清激素方面改变与人类多囊卵巢综合征的病理改变加以比较,探讨睾酮联合HCG是否是建立多囊卵巢大鼠模型的有效方法,并比较两种造模法是否存在异同点,判断哪一种造模方法更为理想及简便有效。

The item adopts biology engineering technique, withdrawing micromolecule peptide , amino acids, glycoprotein etc. from three-month sheep's ovary embryos and whole blood of cyetic horses. The product contains ovarin, sheep embryo and PMSG, these biological ingredients are great demanded by female. It can repair ovary cell, keep hairdressing, defering consenescence and reducing female disease occurrence.

项目采用生物工程技术,对三个月羊卵巢胚胎,孕马血提取小分子肽,氨基酸,糖蛋白等,产品含有卵巢素,羊胎素,PMSG等大量女性需要的生物活性成份,修复卵巢细胞,美容养颜,延缓更年期,减少女性疾病发生。

Results:In this group there were 5 mucinous cystadenoma; 4 serous cystadenoma; 4 teratoma; 2 lager ovarian cyst; 4 subserous leiomyoma of uterine. The massive cystic-solid tumor with irregular cystic wall was seen in mucinous cystadenoma and serous cystadenoma; Teratoma appeared non homogenous density with fat density mass; Subserous leiomyoma of uterine showed large cystic tumor with clear edge, and the size of uterine was normal.

结果:5例卵巢浆液性囊腺瘤,4例黏液性囊腺瘤,CT表现为腹盆腔内巨大囊性或囊实性肿瘤,囊壁厚薄不均;4例卵巢畸胎瘤,CT表现为盆腔巨大肿瘤内部密度不均,可见脂肪密度影;卵巢巨大囊肿2例,CT表现为腹、盆内巨大单囊状肿物;4例浆膜下子宫肌瘤,CT表现为盆腔内巨大囊性肿物,边界清晰,子宫大小正常;1例阔韧带平滑肌瘤,CT表现为密度不均匀的实性肿物,增强扫描强化不均。

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