卵巢的

Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. After 4 weeks intervention, get the left ovarie, a part of hypothalami and pituitary gland from head-off rat, and observe expression of ER, FSHR and GnRH by immune histochemistry method . Then extract RNA from the rihgt ovarie and the rest hypothalami and pituitary gland, so as to measure the expression of GnRHmRNA 、 ERmRNA 、 FSHRmRNA and P 16mRNA by RT-PCR. And the cerebrum were made to homogenate to examine SOD, GSH-PX and MDA.

各组实验动物灌药4周后，断头处死，取部分下丘脑、垂体及左侧卵巢组织固定切片，免疫组化法检测 ER 和 FSHR 表达、 GnRH ；取剩余部分下丘脑、垂体及右侧卵巢组织提取总 RNA ，用于 RT-PCR 检测下丘脑 GnRHmRNA ，下丘脑、垂体的 P 16mRNA 及下丘脑、垂体、卵巢的 ERmRNA 、 FSHRmRNA 基因表达；另外取部分大脑组织制作脑组织匀浆，用于 SOD 和 GSH-PX 酶活力以及 MDA 含量的测定。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。