卵巢癌

It was the first time that using 〓 cell line and normal immunity inbreed line BalB/c mice as objects to construct the subcutaneous transplanting ovarian cancer model. Histopathological experiment proved that the transplanting tumor was undifferentiated cancer. The ovarian tumor marker 〓 and the epithelial tumor marker 〓 were positive in the transplanting tumors. According to these proofs we thought we had already succeeded in constructing animal tumor model.

本实验首次采用卵巢癌〓细胞系，以正常免疫力的雌性近交系BalB/c小鼠为对象，建立了卵巢癌皮下移植瘤模型，经病理组织学检查证实移植瘤为低分化癌，经免疫组化检测证实移植瘤组织中卵巢肿瘤标志物〓和上皮性肿瘤标志物〓表达阳性，据此我们认为动物肿瘤模型建立成功。

We detected the expression of OCY-66, OCY-189 and OCY-142 in 28 ovarian cancers and 30 normal ovarian tissues by RT-PCR, and studied the expression of OCY-142 in these ovarian cancers and normal ovarian tissues by nonradioactive hybridization in situ.

采用RT-PCR和原位杂交的方法研究卵巢癌相关肿瘤抗原新基因在卵巢癌组织和正常卵巢组织中的表达，分析其与卵巢癌临床指标的关系。

Methods SP immunohistochemical staining was used to detect the level of VEGF and EG-VEGF in 85 cases of the epithelial ovarian tumor tissue(25 cases of benign tumor,27 cases of ovarian carcinoma of stage FIGOⅠ、Ⅱ,33 cases of ovarian carcinoma of stage FIGOⅢ、Ⅳ)and 20 cases of normal ovarian tissue.The integral optical density was measured.

方法应用免疫组织化学SP法和形态定量学方法检测85例卵巢上皮性肿瘤(良性25例，FIGOⅠ、Ⅱ期卵巢癌27例，FIGOⅢ、Ⅳ期卵巢癌33例)和20例正常卵巢(除外卵巢疾病的绝经前患者)组织中VEGF、EG-VEGF表达的阳性率及积分光密度值。

The CA125 is an epitope on a large mucin glycoprotein molecule of greater than 1 million Daltons,normal expressed on the coelomic epithelium,including ovarian surface.By now,CA125 assay has been used for monitoring response to primary treatment,detecting clinically occult ovarian cancer at the time of recurrence,as well detecting primary disease in early stage.The CA125 level is abnormal in over 90%of advanced stage ovarian cancer,but it is not increased in about 50%of the patients with stageⅠdisease.

卵巢癌肿瘤标记物中最常用的是CA125，它是体腔上皮正常表达的一种抗原蛋白，晚期卵巢癌中，超过90%病例CA125升高，而在Ⅰ期卵巢癌中，大约有50%病例CA125正常，因此血清CA125敏感性和特异性不能满足筛查指标的要求。

Methods Semi-quantitative RT-PCR was employed to analyze the polymerase β mRNA in 41 cases of epithelial ovarian carcinoma, 27 cases of serous cystadenocarcinoma, and 4 cases of mucinous cystadenoma, endometrioid tumor, and 31 cases of normal ovarian tissue.

采用含有内对照的半定量RT-PCR方法，检测41例卵巢癌患者（其中浆液性囊腺癌21例，黏液性囊腺癌13例，子宫内膜样癌7例）及正常卵巢组织31例pol βmRNA的表达。

The joint inspection of CA125,SF and CA199 can improve the positive rate of diagnosis of oophoroma.

CA125、SF和CA199联合检测可提高卵巢癌诊断的阳性率，对卵巢癌的早期诊断有一定的价值，而增加CEA检测不能提高卵巢癌检出率。

And it has some value for the early diagnosis of oophoroma.However, increasing CEA inspection cannot improve the detectable rate of oophoroma.

CA125、SF和CA199联合检测可提高卵巢癌诊断的阳性率，对卵巢癌的早期诊断有一定的价值，而增加CEA检测不能提高卵巢癌检出率。

Methods Expression of p16 was examined in 30 cases of normal ovarium, 32 benign ovarian tumor, 8 borderline ovarian tumor and 103 ovarian cancer by using ABC immunohistochemistry. The association between p16 expression and clinicopathology was also analyzed.

用免疫组化 ABC法检测30例正常卵巢、32例卵巢良性肿瘤、8例交界性卵巢肿瘤和 10 3例卵巢癌组织中的抑癌基因 p16蛋白表达情况，分析 p16蛋白表达与卵巢癌分级分期、转移预后的关系。

Down-regulated expression of PTEN and up-regulated expression of VEGF〓 were considered as two important events in tumorigenesis of ovarian cancer and could act as molecular markers to indicate the pathobiological behaviors of ovarian cancer. Decreased PTEN expression and increased VEGF〓 expression were closely associated with tumorigenesis and pathobiological behaviors of ovarian endometrioid and serous cancer respectively. Reduced expression of PTEN gene might be involved in carcinogenesis and progression of ovarian cancer by up-regulating the VEGF〓 expression to enhance angiogenesis.

在卵巢癌组织中PTEN基因mRNA表达下调和VEGF〓基因mRNA表达上调是卵巢癌发生的重要分子事件，可作为反映卵巢癌病理生物学行为的指标，且分别与卵巢内膜样癌和浆液性囊腺癌的发生和病理生物学行为关系密切；PTEN基因mRNA表达降低可能通过上调VEGF〓基因mRNA表达促进新生血管形成，进而参与卵巢癌的发生和侵袭。

It is useful to understand the relationship between the structures and functions of the ABC transporters and drug resistance and the prognosis of ovarian cancer patients for individualizing treatment in patients.

了解ABC转运体的结构、功能以及与卵巢癌耐药和患者预后的关系，为卵巢癌患者的个体化治疗提供理论依据，这也成为目前研究卵巢癌耐药机制的热点。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。