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Immune POF model elicited by immunization in subcutaneous with antigen sourced isotype animal ovaries can cause growing follicles and mature follicles decrease obviously, the morphology of oocytes and zona pellucida abnormal, the adjacent granular cells disorder and atresic follicle increase.

以同种动物卵巢为抗原主动免疫建立的免疫性POF模型可致生长卵泡和成熟卵泡数明显减少,卵母细胞及透明带形态异常,周边颗粒细胞排列紊乱,闭锁卵泡增多,病理损伤随免疫时间延长而加重。

Pathology slices showed increased counts of primordial follicles and primary follicles, while the counts of secondary follicle and mature follicle was decreased in high dosage group. In addition, there were more atretic follicles.

高剂量染毒组大鼠卵巢组织的病理改变主要表现为始基卵泡和初级卵泡增多,而次级卵泡和成熟卵泡较少见,且闭锁卵泡增多。

The rates of apoptotic granulosa cells in all kinds of follicles in immature gilts were ashigh as atretic follicles;it decreased significantly after injection of PMSG,but it was higherand lasted shorter than that of mature pig.

性未成熟仔母猪卵巢中各类卵泡颗粒细胞凋亡比例很高,接近闭锁卵泡的凋亡比例;注射PMSG后则各类卵泡颗粒细胞凋亡比例显著下降,但高于性成熟母猪注射PMSG后的凋亡比例,并且维持的时间也较短。

E. staining and other techniques were used to elucidate the pattern of follicular development, atresia, changes in follicle number on the ovarian surface, proportions of apoptotic granulosa cells in follicles of different sizes and morphological changes of atretic follicles, as well as changes in serum estradiel and progesterone levels at different stages of the porcine estrous cycle.

为探讨猪发情周期不同时期卵泡发育和闭锁的规律及机理,本实验通过TUNEL原位标记、H.E。染色以及放射免疫测定等手段研究了猪发情周期不同阶段卵巢表面各类卵泡数量的变化、各类卵泡中颗粒细胞的凋亡比例、闭锁卵泡的形态变化以及发情周期各阶段血清孕酮及雌二醇水平的变化等问题。

Most of photos show an atretic follicle with central vascular structure, a layer of atrophic and splindle granulosa cell layer and a somewhat lutinized theca interna layer which is merging with ovarian stroma.

多数照片显示为具中央脉管结构的闭锁卵泡,一层营养层加上梭形粒层细胞以及被卵巢间质吸收融合的某些黄素化卵泡膜内层。

Brachymystax lenok ; ovary ; follicular cell ; atretic cells

细鳞鱼;卵巢;滤泡细胞;闭锁细胞

Methods: A dual-chamber culture system was prepared. Porcine ovarian follicles were divided into healthy and atretic groups. Granulosa and thecal cells were isolated, and cultured with gonadotropin in the dual-chamber system. After 48 hours, angiotensin II, renin activity, estrodiol(E2), progesterone and testosterone in the media were determined by radioimmunoassay.

制备人胎盘羊膜双池培养系统,分离了猪卵巢颗粒细胞和卵泡膜细胞,并分为健康和闭锁两组,在促性腺激素刺激下协同培养。48 h后应用放射免疫测定法测定两种细胞培养液中的血管紧张素II、肾素活性、雌二醇(E2)、孕酮以及睾酮水平。

The vast majority of follicles undergo a degenerative process in reproductive life. Apoptotic cell death is the fundamental mechanism in response to follicular atresia. Moreover, the apoptosis of granulose cells is implicated in directly mediating atretic process. Multiple studies have confirmed that the apoptotic process of GCs is not only related to apoptotic genes, but triggered by cytokines related to reproduction and Caspases family.

中文题名几种生殖激素对体外培养的牦牛卵泡颗粒细胞凋亡的影响副题名外文题名 Effects of several reproductive hormones on apoptosis of ovarian granulose cells of yaks in vitro 论文作者杨世华导师余四九教授学科专业临床兽医学研究领域\研究方向学位级别博士学位授予单位甘肃农业大学学位授予日期2002 论文页码总数108页关键词牛类固醇激素分泌卵泡颗粒细胞培养牦牛生殖激素馆藏号BSLW /2003 /S823 /1 卵泡闭锁是哺乳动物卵巢中极为普遍的自然现象。

Carbon dioxide has auxoaction on the growth of the human ovarian carcinoma cell line SKOV3 xeno-transplanted into nude mice, but has no effects on tumor metastasis.

CO2气体本身对人卵巢癌SKOV3细胞裸鼠皮下移植瘤的生长有明显的促进作用,对转移影响不大。

Carbon dioxide has auxoaction on the growth of the human ovarian carcinoma cell line SKOV3 xenotransplanted into nude mice, but has no effects on tumor metastasis.

结论CO2气体本身对人卵巢癌SKOV3细胞裸鼠皮下移植瘤的生长有明显的促进作用,对转移影响不大

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