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Results :3 cases Simple ovarian cysts , 3 cases postoperatively diagnosed as endometriotic cyst , 7 cases teratoma , 7 cases crinoid cysts , 7 eases corpus luteum cysts , 1 case brenner tumor , 1 case mucinous cystadenoma and 1 case ovarian mutinous syst, 8 parovarian.

结果:术后诊断单纯性卵巢囊肿3例,卵巢巧克力囊肿3例,其中2例为双侧卵巢巧克力囊肿,卵巢成熟畸胎瘤7例,卵巢黄体囊肿7例,卵巢冠囊肿7例,卵巢膜纤维瘤,卵巢粘液性囊昧瘤及卵巢粘液性囊肿各1例,输卵管系膜囊肿8例。

Vg could be detected in haemolmph of the water—fed female at 48h after eclosion, too.

用10%的蜂蜜和清水分别饲喂两组成虫(简称"蜜组"和"水组"),以研究成虫期的营养对Vg发生和卵巢发育的影响,结果显示,成虫期营养对于Vg的启动合成并不起关键的决定性作用,两组成虫均可以在羽化的48小时检测到Vg,且水组中的血淋巴水平并不低于蜜组对照,但绝大多数水组个体的卵巢发育停留在卵黄沉积的某一时期,此时在血淋巴中也检测不到,只有约20%的饲水个体卵巢可以发育成熟,出少量卵;用高剂量的ZR515于羽化当日点滴处理饲水个体,可以使所有接受处理的饲水个体的卵巢发育成熟,但是产卵〓依旧低于对照组的,不能恢复正常;这说明成虫期营养对卵巢中全体卵母细胞的成熟是必需的,其作用的产生是通过内分泌系统途径实现的。

Methods SP immunohistochemical staining was used to detect the level of VEGF and EG-VEGF in 85 cases of the epithelial ovarian tumor tissue(25 cases of benign tumor,27 cases of ovarian carcinoma of stage FIGOⅠ、Ⅱ,33 cases of ovarian carcinoma of stage FIGOⅢ、Ⅳ)and 20 cases of normal ovarian tissue.The integral optical density was measured.

方法应用免疫组织化学SP法和形态定量学方法检测85例卵巢上皮性肿瘤(良性25例,FIGOⅠ、Ⅱ期卵巢癌27例,FIGOⅢ、Ⅳ期卵巢癌33例)和20例正常卵巢(除外卵巢疾病的绝经前患者)组织中VEGF、EG-VEGF表达的阳性率及积分光密度值。

Forty adult female rats were divided randomly into 4 groups:① Pseudo-operation control group: no ovariotomy, no common carotid artery occlusion;② Cerebral ischemia reperfusion group: with CCAO only, no OE;③ Ovariotomized control group: with OE and CCAO, no estradiol;④ Estradiol supplement group: with OE, CCAO and estradiol supplemented (30 d after ovariotomy, estradiol was supplemented every day for 14 d). All the rats were sacrificed after 12h of reperfusion injury, 10% of brain tissue was homogenized and 10% alue was determined by UV spectrophotometer, NO content was detected, and frozen sections and NOSmRNA in-situ hybridization were performed.

取SD大鼠40只随机分为4组:①假手术对照组:不切除卵巢,不夹闭颈总动脉;②脑缺血再灌注组:作局灶性脑缺血再灌注手术,不切除卵巢;③去卵巢对照组:切除卵巢,作局灶性脑缺血再灌注手术,不补充雌激素;④补充雌激素组:切除卵巢,作局灶性脑缺血再灌注手术,补充雌激素(切除双侧卵巢30d开始补充雌激素,连续2周)。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术,建立了对虾二倍体和三倍体卵巢间的2个消减文库;在正向消减文库(以三倍体卵巢作为实验组,二倍体卵巢作为驱动组)中,鉴定到54个基因;在反向消减文库(以二倍体卵巢为实验组,三倍体卵巢为驱动组)中,鉴定到16个基因;选取11个差异表达的基因,利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测,均能很好地与消减结果相吻合;这些差异基因编码多种功能的蛋白,分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响;为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

Methods Fetal ovaries were transplanted to rats who had received unilateral ovarian extirpation or oophorotomy, and then the interaction between local ovaries and transplanted ovaries was detected.

将胚鼠卵巢分别移植至单侧卵巢摘除及去势雌鼠皮下,观察同时存在的自身卵巢对移植卵巢生长发育有无影响,以及自身卵巢是否受移植卵巢影响。

The positve rates in serous carcinomas and borderline tumors were 39.3% and 55.6%, significantly lower than that in benign tumors and normal ovaries (P.05), but the statistical differences were not found between borderline tumors and serous carcinomas, benign tumors and normal ovaries.2 WWOX mRNA and protein expression in ovarin seruous tumors2.1 WWOX mRNA expression in ovarin seruous tumors The result of RT-PCR showed that WWOX mRNA expression were detected in 29 of 32 ovarian serous benign tumors, 12 of 16 ovarian serous borderline tumors, and 21 of 45 ovarian serous carcinomas respectively.

阳性条带密度扫描定量结果,卵巢浆液性癌中PTEN蛋白表达相对量为0.34±0.12比正常卵巢和浆液性囊腺瘤和交界性囊腺瘤明显降低(p.05),其余各组间无统计学差异。3.2.2 PTEN蛋白FCM检测结果FCM结果,卵巢浆液性癌PTEN蛋白表达的FI值(0.908±0.023)明显低于正常卵巢卵巢囊腺瘤和交界性囊腺瘤的FI值,差异有统计学意义(P.05)。3.2.3 PTEN蛋白免疫组织化学结果35例浆液性囊腺瘤、18例交界性囊腺瘤和56例浆液性癌中,分别有31例、15例和29例呈阳性表达。

Results 531 cases postoperatively diagnosed as endometriotic cyst,254 cases teratoma,139 inflammatory masses,121 serous cystademona,67 cases mucinous cystadenoma,128 parovarian,140 simple ovarian cyst,3 cases brenner tumor,1 case borderline mucinous cystadenoma,1 case mucinous cystadenocarcinoma,1 case endometrioid carcinoma and 1 case malignant brenner tumor.

结果术后诊断卵巢内膜异位囊肿 5 3 1例、卵巢畸胎瘤 2 5 4例、炎性肿块 13 9例、卵巢浆液性囊腺瘤 12 1例、卵巢粘液性囊腺瘤 67例、输卵管系膜囊肿 12 8例、卵巢单纯性囊肿 14 0例、卵巢纤维上皮瘤 3例、粘液性交界性囊腺瘤 1例、粘液性囊腺癌 1例、卵巢子宫内膜样癌 1例、卵巢恶性纤维上皮瘤 1例。

Ovarian stromal flow PSV, EDV and perifollicular vascularity are highly interrelated with ovarian response. Ovarian response will be improved when ovarian blood velocity increases and perifollicual blood flow is abundant. Follicular fluid VEGF level was obviously higher in poor responder group, which suggests VEGF be secreted increasing for compensating due to reduced ovarian stromal blood velocity and fewer perifollicular vascularity.

卵巢基质内血流的PSV、EDV和卵泡周血流分布、卵泡液VEGF水平与卵巢的反应性密切相关,卵巢基质内血流速度升高、卵泡周边血流丰富,有助于提高卵巢的反应性;低反应卵巢组卵泡液VEGF水平显著升高,推测是由于其卵巢基质内血流速度低,卵泡周血管分布少,导致VEGF代偿性分泌增加。

The snails used for this study, measuring within the range of 7.1 to 3.0mm., were collected from the banks of Ching-San River, Chungking, Kiangsu.

从11月到第二年5月,卵巢呈丰满而多卵的状态;6月卵巢趋向萎缩;7、8月卵巢萎缩,内仅含有少量的幼稚卵细胞;9月卵巢开始趋向丰满;10月卵巢逐渐恢复丰满的状态。卵巢全年周期变化的过程与镇江地区钉螺的产卵情况基本上相符合。

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