卵囊

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

To analyze effect of diclazuril and maduramycin on 18S rDNA of Eimeria tenella, the 18S rDNA of sporulated oocyst of the E. tenella anti-diclazuril strain, the E. tenellaanti-maduramycin strain and the E. tenella drug-sensitive strain were cloned and sequenced, and were compared by using DNAStar 5.0 and RNADraw 1.1, respectively.

为探讨抗球虫药物地克珠利和马杜拉霉素对柔嫩艾美球虫18S rDNA的影响，分别对人工诱导的柔嫩艾美球虫地克珠利抗药株、马杜拉霉素抗药株和药物敏感株孢子化卵囊的18S rDNA基因进行了克隆测序，并通过生物信息软件进行对比差异分析。

Chicken (n=50) were randomly grouped in control, control, Bt crystal proteins- treated, Diclazuril-treated, and Amprolium-treated group. GroupⅡ,Ⅲ,Ⅳ, andⅤwere inoculated with 10×104 sporulated E. tenella PT0705 occysts per bird. The results showed that the anticoccidia indexes of the Bt crystal proteins,Diclazuril and Amprolium were 202.3,184.1 and 128.2 respectively.

将50只雏鸡随机均分为5组，Ⅰ组为无感染不用药空白对照组，Ⅱ-Ⅳ组各鸡均感染，每只鸡人工感染柔嫩艾美耳球虫PT0705卵囊10×104个，Ⅱ组为不用药对照组，Ⅲ组为苏云金杆菌晶体蛋白组，Ⅳ组为地克珠利组，Ⅴ组为氨丙啉组，观察效果。

Ten 10-day-old artificially-reared coccidian-free goslings were inoculated orally with 2.0×105-6.5×105 of sporulated oocysts of Eimeria stigmosa,respectively, and killed at intervals from 48th h to 180th h post-inoculation.

给10只10日龄雏鹅分别经口接种2.0×105~6.5×105个多斑艾美球虫孢子化卵囊，在接种后48~180h分期剖杀，对多斑艾美球虫的生活史和致病性进行了动态观察。

An insect that feeds on hemlocks; its egg sacs are small fuzzy white balls like artificial snow on a Christmas tree.

以铁杉为食的昆虫；其卵囊为有绒毛的小白球，形似圣诞树上的人造雪。

The sacs may be carried around on the spinnerets, between the legs or in the jaws.

卵囊也可能被放在吐丝器上，肢体间或颌中跟随蜘蛛。

The sacs may be carried around on the spinnerets, between thelegs or in the jaws.

卵囊也可能被放在吐丝器上，肢体间或颌中跟随蜘蛛。

Silken egg sacs may be hidden in vegetation, or in crevices, or buried in the ground.

丝质卵囊可能被藏在植物或石缝中，或被埋在地下。

Silken egg sacs may be hidden in vegetation, or in crevices, or buried in the ground.

译文丝质卵囊可能被藏在植物或石缝中，或被埋在地下。

Considering the nutritive and comprehensive utilization of its eggs, this paper deals with the nutritive value and food safety of white wax scale eggs.

从白蜡虫卵的综合利用角度出发，研究了白蜡虫卵和卵囊壳的营养成分和食用安全性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。