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The decoction did not have the influence regarding to the wet weight of uterus.(2) Wet weight of ovary:Compared with the blank group,the ovary weight of other groups were significantly lighter(p<0.05).There is two reasons:First, because of decoction intervention ahead of time,it may had the prevention and the treatment dual function;Second,it possibly promoted the ovulation, and caused the liquid discharged from the pouch of the ovary,thus promoted ovary volume and weight decline.(3) Endocrine changes:①The content of androsterone in high dose groups were significantly lower than the low dose groups(p<0.05),Chinese herbal had certain dose-effect relationship.

上方对于子宫湿重无影响(P>0.05)。2、卵巢湿重:用药的各组与空白组比较,对于卵巢的重量均有影响(p<0.05),考虑有两个原因:第一,由于造模的同时用药,有预防与治疗的双重作用;第二,可能是促进排卵,使囊内卵泡液排出,从而促进卵巢体积及重量的下降。3、内分泌改变:①高剂量组别与低剂量组别比较,在降调总睾酮的作用更大,差异有统计学意义(p<0.05),说明中药治疗作用具有一定的量效关系。

The transcripts of XSox1 are detected in the animal pole at cleavage and blastrula stages and mainly in the central nervous system and the developing eye at neurula stages.

原位杂交结果显示,在早期卵裂阶段和囊胚期,Sox1基因主要在动物极表达;从神经板期开始,Sox1基因主要在中枢神经系统和眼原基中表达。

A independent life individuality is finally formated from singlecell ferti1ized egg throngh egg cleavage、morula stage、blastocoele stage、protogaster stage and orange etc, and concerned in major basal questions of development and gene expression.

单细胞的受精卵通过卵裂、桑椹胚、囊胚、原肠胚及器官的发生等,最后形成一个能独立生活的个体,涉及发育和基因表达的许多基本问题。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示,在卵母细胞和8细胞期胚胎,基因calm3和trm112l的表达量无显著性差异,而在囊胚期的表达量与前两个时期的表达量存在显著性差异,推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控,提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

The results show the Boer goat oocyte and pre-implantation embryo are telomerase positive. According to the densitometry of bands under the CCD imagine system, the total telomere repeats product generated of oocytes, 4-cell, 8-cell, morulae and blastocyst are 25.348, 273.832, 56.117, 251.118, 519.46, respectively.

结果表明波尔山羊卵母细胞和早期胚胎都是端粒酶阳性;依据电泳条带在凝胶成像系统下的光密度计算相对总产品产量定量比较发现,卵母细胞最低为25.348,4-细胞为273.832,至8-细胞又降低到56.117,随后快速升高,桑椹胚为251.111,囊胚端粒酶活性最高为519.46。

This medicine may cause women who have stopped ovulating due to polycystic ovary syndrome to start ovulating again.

这个药可能让那些因为患上多囊卵巢综合征不能排卵的女性重新排卵。

Owing to placenta and milk transshipping effect, the content of Aroclor 1254 in fetal and infant vivo was very low, so there were not significantly pathological changes in sexual gland, the effect of Aroclor

2首次以排卵数、受精率、受精卵的第一次卵裂率、桑堪胚发育率、囊胚~孵化胚发育率、以及发育延迟胚和退化变性胚的比率,对个体发育的不同时期接触不同剂量 AroClor 1254对小鼠卵子发生、受精和附植前胚胎体内发育毒性进行系统定量。

At final stage, the oocyte contained many globules, then developed to the mature parthenogenetic ovum really and moved to the female's brood pouch.

后期的卵母细胞含较多的卵黄颗粒,最后成为成熟卵子,排入孵育囊内形成夏卵。

Early cleavage rate,embryo quality,the rate of blastocyst,implantation and pregnant rates from these zygotes were observed,and the transferred embryos were selected according to the pronuclei orientation and early cleavage time.

根据受精卵原核构型的不同分为四组,结合构型类型及早期卵裂与否进行选择性移植,通过观察优质胚胎率、囊胚形成率、种植率和怀孕率,来评估合子原核构型和早期卵裂与胚胎发育潜力的关系。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明:(1)水牛精子胞质内显微受精的雌、雄原核发育不同步,雌原核比雄原核早3h形成;(2)水牛卵母细胞ICSI和激活后用1.9mmol/L 6-DMAP培养处理3h能提高其胚胎发育率;(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率;(4)死精子能用于水牛卵母细胞的ICSI;(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

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