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卵囊

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The second was to compare the effects of the different floating liquids on the destruction of rabbit coccidia oocysts. The results indicated that magnesium sulfate solution showed the lowest rate of coccidia oocyst destruction at 20 min; saturated salt water had the greatest rate of destruction. The destruction increased with time; in the saturated salt water and urea solution damage or deformation of a few coccidia oocysts at were observed 60 min.

其二,不同漂浮液对兔球虫卵囊破坏作用比较;结果表明20min时硫酸镁溶液对卵囊破坏率最低,破坏率最大的是饱和食盐水,随着时间延长各种破坏作用增大,60min时饱和食盐水和尿素溶液中有极少卵囊破损和卵囊变形现象。

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

在感染后72 h内,隐孢子虫出现连续发育阶段,包括脱囊、子孢子、裂殖子、裂殖体、滋养体、配子体、合子、薄壁卵囊和厚壁卵囊,在60~72 h内形成卵囊;用感染48 h的细胞培养上清接种于免疫抑制小鼠, 10 d后有隐孢子虫卵囊排出。

The snail was found to have an indirect developmental type in the early development. The fertilized eggs were oval-shaped and discoidal cleavage. Embryonic development stages were divided into cleavage, blastula, gastrulae, trochophore, and intra-membrane veliger. Larval development stages included veliger, late veliger and crawling larvae metamorphosed to juvenile.

结果表明:黄口荔枝螺卵囊高度平均为7.33 mm,卵粒数量平均为165粒;黄口荔枝螺的早期发育属间接发生型,受精卵呈椭圆形,卵裂为盘状卵裂;在卵囊内,胚胎发育包括卵裂期、囊胚期、原肠胚、膜内担轮幼虫、膜内面盘幼虫;幼虫发育包括面盘幼虫、后期面盘幼虫和匍匐幼虫。

In Hangchow it has four generations each year and the time of its occurrence is well synchronized with that of the scale.

根据测定,隐斑瓢虫对日本松干蚧各虫态的捕食能力较强,一头成虫的日平均捕食量为:显露若虫13.33头,雌成虫4.27头,卵囊3.67个(每个卵囊内平均有卵265.5粒),雄蛹28.27只;一头幼虫全期(或2—4龄和3—4龄)分别能捕食显露若虫39.20—105.56头,雌成虫40.80—47.79头,卵囊39.38—41.79个,雄蛹220.50只。

Objective To study the change of intracellular free Ca2+ in the oocyst when it melanized and to find out the relationship between the melanized oocyst and its intracellular level of free Ca2+ in a Plasmodium-refractory strain of Anopheles dirus.

目的研究大劣按蚊蚊胃的约氏疟原虫卵囊黑化时卵囊内Ca~(2+)的变化,探讨疟原虫感染不易感蚊种时卵囊黑化与囊内Ca~(2+)的相互关系。

Results 4.16×10 9 oocysts were obtained in 30 mice in the 3rd group with dexamethasone of 20 μg/ml in drinking water. No significant difference in the oocyst recovery, purity and infectivity was found between methods using saturated saline floatation and sucrose density gradient centrifugation. The infectivity of the oocysts obtained from the same origin but different animals was similar.

结果 地塞米松加入量为 2 0 μg/ml的第 3组获得的卵囊量高达 4 。16× 10 9/ 30个小鼠;用饱和盐水漂浮法与用蔗糖密度梯度离心法纯化得到的卵囊具有相同的回收率和纯度;从感染小鼠与犊牛中获得的卵囊在感染性上差异不显著。

Results In the aspect of oocyst intensity discharged from mice, femal BABL/C mice were more than the male, the 50 day-old mice(26 g~28 g) were more than the 25 and 75 day-old, mice in 15 mg/L group were more than those in the and mice infected after immunosuppressed for 3 days were more than those infected after immunosuppressed for –6,-3,0,6,9 days.

结果 雌性小鼠排卵囊强度均较同日龄雄性小鼠大,而不同日龄小鼠中又以50日龄(26 g~28g)小鼠排卵囊量最多;各剂量组小鼠中,15 mg/L组排卵囊强度较其他各组大,10 mg/L组小鼠次之;免疫抑制时间各组中,免疫抑制3天后感染的小鼠排卵囊强度较其他组大。

KM strain mice immunosuppressed with 7. 5~10mg/L DEX in drinking water had the higher oocysts output and fewer dead than other groups.(4) There was no significant difference infection rate between groups inoculated with different number CSO and groups inoculated CSO with conserved different time. Trial were divided into control, groups of 50mg/kg /d, 100 mg/kg /d and 200 mg/kg /d dose of the drug to study the anticryptosporidial activities of nitazoxanide in an immunosuppressed mice model. The experimental results showed the treatment with 200mg/kg bw /day was significant inhibition of oocyst excretion. To detect the two cryptosporidiums strain C.

实验结果表明:C.parvum虫种能够感染上本实验所采用的BALB/c和昆明小白鼠两种小鼠,而C.andersoni没有能够感染本次实验小鼠;且从小鼠感染后的排卵囊量和小鼠的生存状态来看,采用5~6周龄的KM鼠能获得最大量的隐孢子虫卵囊,小鼠的死亡数最少;每升饮水中地塞米松量为7.5mg~10mg组比12.5mg以上组小鼠死亡数量少,比5mg/L以下组排卵囊数量多;接种不同卵囊数和卵囊的保存时间这两个参数均对小鼠受感染后的排卵囊情况影响不大。

Based on the security,efficacy,cheapness and easy gain three disinfectant drugs(acids, degerming agents,surface active agents)were chosen to killing coccidial oocysts in chicken.

本试验按照安全、有效、经济、来源容易的原则,从对鸡球虫卵囊有一定抑杀作用的三类消毒药物(酸类、消毒剂类、表面活性剂类)中,筛选了 17组不同药物和浓度的搭配组合,设计了 3个试验:抑制鸡球虫新鲜卵囊孢子化试验;抑杀孢子化卵囊试验;新鲜卵囊和孢子化卵囊经药物处理后感染小鸡试验。

The results showed that among the 3 NO donors,only GSNO had significant effects on the percentage of sporulated oocysts,theinhibiting rate of purified sporulated oocysts was 93% to 97%,and reached 100% onthe oocysts in the faces of chicken with E.tenella infection.GSNO also significantlydecreased virulence of E.tenella oocysts;and the oocysts treated by 2~8 mmol/LGSNO almost lost their pathogenicity to chickens.

试验结果表明,在以上3种NO供体中,只有GSNO对卵囊的孢子化率有明显的抑制作用,其对纯化卵囊的抑制率为93%~97%,对粪便中卵囊的抑制率可达100%;GSNO还能明显抑制E.tenella卵囊的毒力,经2~8mmol/L浓度GSNO处理的卵囊几乎丧失了对雏鸡的致病力。

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