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Interestingly, a monopolar spindle was assembled in each blastomere in the second cell cycle, and disjunction of duplicated chromosomes and the cleaage was preented, leading to the chromosome set doubling.

有趣的是,在第二个细胞周期中,每一个分裂球中都组装了一个单极纺锤体,同源染色体分离和卵裂被阻止,导致染色体组加倍。

The code plate is equipped with intelligence code, energy code, constitution code, ovulation conclusion table of women with normal catamenia as well as code positioning cooperation method.

8 ,其组成包括衬盘、生理周期盘、代码定位盘和标尺,生理周期盘、代码定位盘和标尺分别配装在衬盘的正、反两面,由盖拴固定在衬盘上,生理周期盘的月日盘周圈上设有月日相应标识,精力盘、智力盘和体力盘周圈上分别设有精、智和体力生理周期循环变化标识,内圈盘依次位于相邻外圈盘上标识内圈:代码定位盘的年份盘周圈上设有 00-99 个年份标识,代码盘上设有智力代码、精力代码、体力代码标识和月经正常女性排卵时间推算表以及代码定位操作方法;标尺为两把,分别配装在生理周期盘和代码定位盘上,生理周期盘上的标尺上设有指示标尺线,代码定位盘上的标尺设有定位代码标识框;本实用新型优生测算器结构简单,测算内容显示直观,操作简便。

Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组:早期RA家兔模型15只,品种及体重接近。对照组:正常家兔10只,品种及体重接近。RA家兔模型制作方法:选取15只成年家兔,体重、品种接近,将卵蛋白溶解于生理盐水,配成浓度为20mg/ml的溶液,与等量完全弗氏佐剂混匀,注入家兔肩胛区皮下致敏,每只家兔每次注射1ml,于肩胛区5个不同区域注射,每周一次,连续注射3周而致敏,第4周向膝关节腔注射卵蛋白生理盐水溶液,每只关节腔注射5mg卵蛋白,15只家兔膝关节全部注射,24h内此关节直径和表面温度大幅度上升,以后缓慢下降,至14~21d达到平台期,发病率达100%。造模成功后第1~4周为早期改变,第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组,其股骨髁软骨的厚度与对照组相比无明显差异,其软骨下骨皮质与对照组相比无明显改变。

Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇/卵磷脂/十八胺脂质体包裹,免疫2周龄SPF鸡,4周后用同型禽流感病毒进行人工感染,1周后采集泄殖腔分泌物分离病毒,结果未免疫组6/6分离到病毒,裸质粒DNA免疫组1/6分离到病毒胆固醇/卵磷脂/十八胺脂质体包裹DNA免疫组1/6分离到病毒,DC-chol脂质体DNA免疫组没有分离到病毒(0/6):人工感染后1周各组的HI抗体(Log2)分别为6.38±0.98,7.00±1.26,7.83±1.17,8.00±0.89,2周后为8.50±0.55,8.67±0.82,8.68±0.45,9.33±0.52,脂质体包裹组在同期均高于未免疫组和裸DNA免疫组,表明脂质体包裹质粒DNA免疫动物后,能增加动物对病毒感染的抵抗力和反应能力。

The life of a worker honeybee is even separated into successive occupations: during the first three weeks the young worker grooms the queen and her eggs, cleans out the hive, cools it by wing-fanning at the entrance, and attacks or walls in intruders.

工蜂的一生甚至先后分别承担几种工作:最初三周的幼蜂负责照料蜂王和峰卵,清洁蜂巢,在入口处扇动翅膀为蜂巢降温,攻击或围歼入侵者。

To identity the strains of Eimeria conserved in our laboratory are purebred,11 strains of 6 Eimeria species were separated by single-oocyst method and infected chicken of 1—5 days old.

为了建立鸡球虫纯株,选用1~5日龄雏鸡,采用琼脂块单卵囊分离法对实验室保存的6种11株鸡球虫进行纯化,并选用2周龄雏鸡,对每个虫种/株选取1个单卵囊分离物进行增殖和种类鉴定。

Crucially, each volunteer was examined twice in a month: once early in her menstrual cycle, before she had ovulated, and once late in the cycle, after ovulation but before the onset of menstrual bleeding.

更为关键的是一周要对每位志愿者做两次检查:一次是在月经初期,排卵之前。另外一次是在月经末期,排卵之后但在流血之前。

Understanding the role of cumulus oophorus in oocyte maturation will enable us to reveal the mechanism of mammal ooc.

卵丘是指在卵母细胞外周并与之进行代谢联系的颗粒细胞群;卵丘对于卵母细胞成熟有极其重要的作用。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母细胞时,将同周期成熟卵丘复合体切出部分卵丘细胞,用1 mL注射器抽打分散细胞,贴壁培养。113个治疗周期中,298枚生发泡期卵母细胞经3种不同培养液体外成熟培养(同一病人的生发泡期卵被随机分到某同一组中):第1组28个周期中73枚:基础培养液+卵泡液;第2组40个周期中115枚:A液+分散贴壁的卵丘细胞;第3组45个周期中110枚:A液+分散贴壁的卵丘细胞+促卵泡生成激素+表皮生长因子。

It is known that ovulation and fertilization triggers embryonic development and endometrial differentiation by corpus luteum progesterone production.

在排卵周期中,子宫内膜仅在极短的时间内才允许胚泡植入,称为种植窗期,通常在月经周期的20-24d,即LH高峰后的第7-11d。

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