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In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达,结果显示:在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达,而在成体的体壁组织、消化道组织、体腔球中则没有表达;在未受精卵中没有表达,而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达;在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

The liver oval cell line WB-F344 derives from adult normal Fisher 344 male rats. It's a kind of liver infantine epithelial cells and has characteristics of liver stem cells. Many studies in vitro and in vivo imply that it has the role as precursor cells of hepatocacinoma, which indicates its function in hepatocarcinogenesis.

肝卵圆细胞株WB-F344来自正常成年同基因Fisher344雄性大鼠,是一种肝脏幼稚上皮细胞,具有肝脏干细胞的性质,体内、外的许多研究表明,它与肝癌的发生有一定的关系,具有肝癌前体细胞的作用。

The liver oval cell line WB-F344 derives from adult normal Fisher 344 malerats.It's a kind of liver infantine epithelial cells and has characteristics of liver stemcells.Many studies in vitro and in vivo imply that it has the role as precursor cells ofhepatocacinoma,which indicates its function in hepatocarcinogenesis.

肝卵圆细胞株WB-F344来自正常成年同基因Fisher344雄性大鼠,是一种肝脏幼稚上皮细胞,具有肝脏干细胞的性质,体内、外的许多研究表明,它与肝癌的发生有一定的关系,具有肝癌前体细胞的作用。

This phenomenon to some extent explains the ability of the migratory locust to withstand low temperature.

特别是在北方地区,越冬蝗卵要在土壤中度过 7个多月的时间,它们能否抵抗住生存环境的寒冷,这对下一季节的发生是至关重要的。

A proportion of the triadiate cells could shorten one arm and gradually pass, during successive divisions into the fusiform cells, however there was little to suggest that fusiform cells ever became triradiate by growing out a third arm.

培养液比重、温度及光照条件的改变短期内对细胞形态类型的转变没有明显影响;淡水和低温(4℃~8℃)的长期胁迫能使梭形和三出放射形藻株发生高比例的卵形细胞。

This procedure can be influenced by a lot of factors, such as hormones, growth factors, et al. Only very few follicles will develop into dominant follicles and ovulation and more than 99% turn atresic. Xiang pigs are a world famous breed of the miniature pig mainly distributed in Guizhou and Guangxi provinces of China.

同时,卵泡的生长和发育也是一个高度协调的生理过程,受多种因素(激素、生长因子、芳香化、金属离子、物理及化学刺激等)的影响和控制,少数卵泡最终发育为优势卵泡并排卵,99%以上的卵泡都将发生闭锁。

This procedure can be influenced by a lot of factors, such as hormones, growth factors, et al. Only very few follicles will develop into dominant follicles and ovulation and more than 99% turn atresic. Xiang pigs are a world famous breed of the miniature pig mainly distributed in Guizhou and Guangxi provinces of China.

同时,卵泡的生长和发育也是一个高度协调的生理过程,受多种因素(激素、生长因子、芳香化酶、金属离子、物理及化学刺激等)的影响和控制,少数卵泡最终发育为优势卵泡并排卵,99%以上的卵泡都将发生闭锁。

There is n strong association of PFO with paradoxical embolism and ischemic cerebral infarction, and reports on patients with PFO-related cryptogenic stroke after coronary artery bypass grafting and various surgical cases of heart or lung are being increasingly published in recent years.

近年来的研究发现,卵圆孔未闭的存在与反常栓塞、缺血性脑卒中的发生密切相关,与冠状动脉旁路移植术及各种心、肺手术后不明原因的卒中或猝死相关的报道日益增多。

There is a strong association of PFO with paradoxical embolism and ischemic cerebral infarction, and reports on patients with PFO related cryptogenic stroke after coronary artery bypass grafting and various surgical cases of heart or lung are being increasingly published in recent years.

近年来的研究发现,卵圆孔未闭的存在与反常栓塞、缺血性脑卒中的发生密切相关,与冠状动脉旁路移植术及各种心、肺手术后不明原因的卒中或猝死相关的报道日益增多。

Both the proteins components contained in haemolymph, fat body and ovary and the kenetics of the vitellogenin in haemolymph and ovary were analyzed by SDS—polyacrylamid electrophoresis, Ouchterlony plate tests and Rocket—immuno—electrophoresis. The results showed that the vitellogenesis initiated in adult stage and the Vg in haemolymph was detected firstly at 48h after eclosion. The titer of Vg in haemolymph raised rapidly in following 3 days and almost at the same time the vitellin appeared in oocytes. It was taken about 4—7 days after eclosion for the first eggs to grow up to the matured statics and to be laid out.

SDS-PAGE,双向免疫扩散和RIE分析粘虫生殖周期中血淋巴,脂肪体和卵巢中蛋白质谱带以及Vg滴度的变化,结果表明,Vg发生始于成虫期,Vg在血淋巴中的首现时间在羽化的24小时以后,以后连续三天血淋巴中的Vg滴度迅速上升,与此同时,开始沉积卵黄,卵母细胞逐渐发育长大,到成熟排出体外时大约历时4-7天。

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