卵原细胞

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

The results show that the CD29, CD49, CD90 express in the gonium and the primary spermatogenous, but there are not expression in the oocyte, the second spermatogenous and the spermatocyte.

研究结果表明，CD29， CD49， CD90在雌性生殖腺各级卵母细胞中均不表达，位于生殖褶边缘的性原细胞中可见表达；在雄性生殖腺的初级精原细胞中表达，但在成团分布的次级精原细胞、各级精母细胞中均未表达。

In the microinjection experiment of mouse oocyte,the principle of cell position and attitude adjusting technology was tested.

以小鼠卵母细胞为实验材料，进行了原理性实验研究。

Since the altered dorsal-ventral domain was started at early gastrulation and became more obvious at the late gastrulation stage, P5 and P4 both may affect cell fate maintenance through regulating cell migration during gastrulation period.

因为cyp11a1表现下降的鱼卵是在原肠期刚要开始的时候才开始出现不正常腹部组织增大，而且随著时间的增加而更加明显，所以P5和P4可能是藉由影响原肠期的细胞移动进而去影响后来的细胞分化。

Except for hemopoietic progenitor cells, spermatogenous cells and oocyts, there was no expression of hTERT in other somatic cells.

除造血祖细胞、精原细胞和卵母细胞表达外，其他正常体细胞中均不表达hTERT。

A modification of two-step perfusion protocol and isopycnic centrifugation were used to isolate oval cells from liver. Primary oval cells were cutured in LE medium in vitro.

经门静脉灌注消化法和等密度离心法分离卵圆细胞，并在体外进行原代培养。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

为探讨Dynein与卵母细胞减数分裂错误形成的关系，采用小鼠卵母细胞体外培养成熟技术和半定量RT-PCR方法，分别检测Dynein抑制剂-原钒酸钠对卵母细胞成熟率和cyclin B1基因转录水平的影响。

At present, the research mainly concentrates on vitellin purification and characterization, vitellogenin synthesis and hormonal regulation, vitellogenin absorption and processing.

目前，对蜱类卵黄发生的研究主要包括卵黄蛋白的纯化与鉴定，卵黄原蛋白的合成与调控，卵母细胞对卵黄原蛋白的摄取以及转变为卵黄蛋白的分子过程。

In the present study, the immunocytochemical localization of ER of termite gonads were investigated with SABC method.

结果表明，ER在末龄若虫期的卵巢和精巢中都存在，免疫阳性物质定位在分化期和生长期的卵母细胞以及精原细胞中。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论：成骨细胞呈梭形，多突起；细胞核呈卵圆形，偏于一侧；胞体大，胞浆丰富；碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒，某些部位染成黑色，定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞；细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐