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The effect of FSH and IGF-Ⅰ on maturation of COCs and the apoptosis of cumulus.

体外培养COCs过程中,FSH和IGF-Ⅰ对体外成熟以及卵丘颗粒细胞凋亡的影响。

Denude oocytes matured in vitro have a clearly deficiency on fertilization invitro.

3去除卵丘细胞的小鼠卵母细胞在受精时存在着明显的障碍。

Remove of granulose cells before maturation will do no harm to earlyembryonic development derived from SUZI.

3在成熟培养前,卵丘细胞的去除对带下受精胚胎的发育没有影响。

FSH and IGF-Ⅰ inhibited granulose cell apoptosis and increased secretion of progesterone.

FSH和IGF-Ⅰ能够促进COCs的体外成熟,抑制卵丘颗粒细胞的凋亡。

The incidence of CGCs apoptosis can be used as an indicator to predict IVF outcome.

卵丘颗粒细胞凋亡的检测可以作为预测IVF结果的指标。

Objective To investigate CD_(44) and interα-trypsin inhibitor expression in the cumulus-oocyte complex of mice.

目的探讨CD44和间α胰蛋白酶抑制剂在小鼠卵丘-卵母细胞复合体的表达及两者的相关性。

Results Under the light microscope, we had figured out that the mean diameter of wapiti oocyte was 172.958±17.298 μm, and the thickness of cumulus cell and zona pellucida were 67.750±33.107 μm and 13.782±2.516μm respectively.

结果 在光镜下,马鹿卵母细胞的平均直径为172.955±17.295μm,卵丘细胞厚度为67.750±33.107μm,透明带厚度为13.782±2.516μm。

The sperm subzonal insemination on mice denudedoocytes. This part of paper use SUZI method to investigate the effects of zona pellucidaon denuded oocytesfertilization.

二、精子注入去除卵丘细胞的小鼠卵母细胞的体外受精利用透明带下精子注射技术,研究透明带对受精的影响。

The results showed that the Ang II positive staining was observed not only in thecal cells , oocytes and zona pellucida of the follicles at different developmental stages but also in corpora lutea.

结果表明:Ang II分布在不同发育阶段卵泡的内、外膜细胞,卵母细胞及其透明带上,颗粒细胞和卵丘细胞未见着色。

Aims and methods: To investigate the distribution of oncoprotiem, ErbB2 in mouse testis, epididymidis, ovaries, oviducts, oocytes-cumulus cells complex and sperms, immunobistochemical method was used.

目的和方法:用免疫组织化学的方法观察ErbB受体家族成员ErbB2在小鼠睾丸、附睾、卵巢、输卵管、卵母细胞—卵丘细胞复合物和精子上的分布。

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