卵丘

1, cumulus cell had important role on nuclear and cytoplasmic maturation of oocytes.

1，卵丘细胞对卵母细胞的核和胞浆成熟均有重要作用。2。

However, the exact mechanism by which cumulus cells promote oocyte aging is unknown.

我们之前的研究证明卵丘细胞能够促进小鼠卵母细胞老化。

To evaluate the role of mature cumulus cells from oocyte-cumulus complex in in-vitro maturation and establish a new culture technique which is convenient to carry out.

探讨人类成熟卵丘细胞在未成熟卵母细胞体外成熟培养中的作用，并建立一种简易的实施技术。

Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取从屠宰场获取的猪卵巢中的卵母细胞，在加入和不加hCG和PMSG的mTCMl99培养液中分别培养22小时，地衣红染色发现77.6％的卵母细胞达到MII期；去卵丘细胞后在mTBM中用鲜精或冻精解冻后受精6小时，在NCSU23中培养14小时后固定染色发现受精率分别为87.5％和64.1％；电激活后在NCSU23中培养20小时后固定染色发现原核形成率为94.1％。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

Methods A C57BL/6j mouse cumulus cell nucleus 10-12 mm in diameter was inserted into the perivitelline space of an enucleated oocyte.

方法将直径10~12mm的C57BL/6j小鼠卵丘细胞核注射到去核卵母细胞透明带下，构建供体核-卵母细胞复合体。

The feasibility of maturing denuded buffalo oocytes in vitro was explored in this study, so as to provide a model to investigate the mechanisms of oocytes maturation.

主要探讨无卵丘水牛卵母细胞体外成熟的可行性，以便为研究卵母细胞成熟机理提供模型。

Rates of fertilization and morulae/blastocysts in oocytes with fully expanded cumulus were significantly higher than those in oocytes with poorly expanded cumulus.

扩展良好的山羊卵巢卵母细胞的受精率和胚胎发育能力显著高于卵丘扩展差的卵母细胞。

Newly ovulated mouse oocytes were cultured in the CZB medium supplemented with different concentrations of pyruvate before treated for activation or assayed for MPF activity.

我们猜测卵母细胞在不含丙酮酸的条件下老化的更快，并且卵丘细胞可能通过消耗不含葡萄糖的CZB中的丙酮酸从而促进卵母细胞老化。

When oocytes were matured at 37-39℃,the appearance of cumulus cell had protective effect on cleavage and blastocyst rate,the development rate of DOs significantly decreased compared to COCs.

当卵母细胞在37-39℃成熟培养时，卵丘细胞对卵母细胞的发育有一定的保护作用，DOs的发育能力显著低于COCs。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。